食品科学 ›› 2012, Vol. 33 ›› Issue (2): 24-28.doi: 10.7506/spkx1002-6630-201202006

• 工艺技术 • 上一篇    下一篇

响应面法优化微波辅助提取枳壳中总黄酮的工艺

邹建国,刘 飞,刘燕燕,彭海龙,李不悔,傅华强,肖鉴谋   

  1. 南昌大学环境与化学工程学院
  • 出版日期:2012-01-25 发布日期:2012-01-16
  • 基金资助:
    江西省自然科学基金项目(2007GZH1924)

Optimization of Microwave-Assisted Extraction of Total Flavonoids from Fructus Aurantii Immaturus by Response Surface Methodology

ZOU Jian-guo,LIU Fei,LIU Yan-yan,PENG Hai-long,LI Bu-hui,FU Hua-qiang,XIAO Jian-mou   

  1. School of Environment and Chemical Engineering, Nanchang University, Nanchang 330031, China
  • Online:2012-01-25 Published:2012-01-16

摘要: 利用响应面法对枳壳中总黄酮的微波提取工艺条件进行优化。在单因素试验基础上,利用中心组合设计原理及响应面法分析建立二次回归模型。以微波功率、提取时间、乙醇体积分数为自变量,柚皮苷、橙皮苷得率的综合指标为响应值,研究各因素及其交互作用对总黄酮得率的影响。利用模型的响应曲面图及其等高线图,确定微波提取枳壳中总黄酮的最佳工艺参数为枳壳粉碎颗粒度为40~60目、液料比100:1(mL/g)、乙醇体积分数58.8%、微波功率447W、提取时间3.9min,在此条件下,柚皮苷、橙皮苷的提取得率分别达到50.21、4.96mg/g,与理论预测值基本吻合。

关键词: 枳壳, 总黄酮, 微波辅助提取, 响应面法

Abstract: This paper deals with the optimization of the process conditions for the microwave-assisted extraction of total flavonoids from Fructus Aurantii Immaturus using response surface methodology (RSM). Based on one-factor-at-a-time experiments, a quadratic regression model indicating the individual and interactive effects of microwave power, extraction time and ethanol concentration on the yields of naringin and hesperidin was established using a Box-Behnken experimental design. From the response surface and contour plots drawn for the model, the optimal extraction conditions were obtained as follows: size reduction ratio 40-60 mesh, liquid/solid ratio 100:1, ethanol concentration 58.8% (V/V), microwave power 447 W, and extraction time 3.9 min. Under these conditions, the experimental yields of naringin and hesperidin were 50.21 mg/g and 4.96 mg/g, respectively, which were in substantial agreement with the theoretically predicted values.

Key words: Fructus Aurantii Immaturus, total flavonoids, microwave-assisted extraction, response surface methodology

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