食品科学 ›› 2012, Vol. 33 ›› Issue (2): 34-38.doi: 10.7506/spkx1002-6630-201202008

• 工艺技术 • 上一篇    下一篇

无梗五加果多糖纯化方法研究

孟宪军,于 磊,冯 颖,王 阳   

  1. 1.沈阳农业大学食品学院 2.沈阳市产品质量监督检验院
  • 出版日期:2012-01-25 发布日期:2012-01-16
  • 基金资助:
    辽宁省博士科研启动基金项目(20091068)

Purification of Polysaccharide from Fruits of Acanthopanax sessiliflorus Seem

MENG Xian-jun,YU Lei,FENG Ying,WANG Yang   

  1. 1. College of Food, Shenyang Agricultural University, Shenyang 110866, China; 2. Shenyang City Product Quality Supervision and Inspection Institute, Shenyang 110003, China
  • Online:2012-01-25 Published:2012-01-16

摘要: 通过树脂法与传统无梗五加果多糖纯化方法的比较,对树脂法纯化无梗五加果多糖展开进一步研究,筛选出树脂HPD-600和聚酰胺并进行纯化比较。结果表明:树脂HPD-600的蛋白去除率、脱色率和多糖损失率分别为66.7%、82.1%、29.8%;聚酰胺的蛋白去除率、脱色率和多糖损失率分别为75.6%、88.1%、33.7%。树脂法对无梗五加果多糖的脱蛋白、脱色效果优于其他方法。

关键词: 无梗五加多糖, 纯化, 大孔吸附树脂

Abstract: The objective of the current research was to develop a resin adsorption method to purify polysaccharides from Acanthopanax sessiliflorus Seem fruits. A comparison of resin adsorption with traditional purification methods was carried out, and the results showed that resin adsorption was the best method. The selected macroporous adsorption resins, HPD-600 and polyamide, were compared for their adsorption and desorption characteristics towards polysaccharides from Acanthopanax sessiliflorus Seem fruits. The deproteinization rate, decolorization rate and polysaccharide loss rate obtained from HPD-600 purification were 66.7%, 82.1% and 29.8%, respectively, and a deproteinization rate of 75.6%, a decolorization rate of 88.1% and a polysaccharide loss rate of 33.7% were obtained using polyamide for polysaccharide purification.

Key words: Acanthopanax sessiliflorus Seem, polysaccharide, purification, macroporous resin

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