食品科学 ›› 2012, Vol. 33 ›› Issue (2): 43-47.doi: 10.7506/spkx1002-6630-201202010

• 工艺技术 • 上一篇    下一篇

超临界体系酶催化制备甘油二酯及其纯化

陈晓慧,王 雪,刘 晶,周晓丹,于殿宇   

  1. 东北农业大学食品学院
  • 出版日期:2012-01-25 发布日期:2012-01-16
  • 基金资助:
    国家“863”计划项目(2010AA101503);“十二五”国家科技支撑计划项目(2011BAD02B01)

Lipase-Catalyzed Synthesis in Supercritical System and Purification of Diacylglycerol

CHEN Xiao-hui,WANG Xue,LIU Jing,ZHOU Xiao-dan,YU Dian-yu   

  1. School of Food Science and Technology, Northeast Agricultural University, Harbin 150030, China
  • Online:2012-01-25 Published:2012-01-16

摘要: 以二氧化碳为流体,在超临界体系中用脂肪酶催化大豆油脂与甘油反应制备甘油二酯及其纯化研究。选取Lipozyme RMIM、Novozyme 435、Lipozyme TLIM 三种固定化脂肪酶为试验酶进行酯化反应,通过单因素试验,分别确定3种酶的最佳工艺条件,3种酶的最佳添加量分别为2.5%、3%、8%;反应温度分别为65、70、65℃,反应时间分别为7、8、9h,底物比均为2:1,得甘油二酯含量分别为68.6%、67.7%、64.8%。采用二级分子蒸馏工艺对超临界体系生产的甘油二酯混合物进行纯化,甘油二酯产品纯度从68.6%提高到90.4%,产品得率为60.0%。综合考虑,在工业生产中,建议用脂肪酶Lipozyme RMIM。

关键词: CO2超临界, 脂肪酶, 甘油解, 甘油二酯, 分子蒸馏

Abstract: Diacylglycerol (DG) was prepared through lipase-catalyzed reaction between soybean oil and glycerol in the supercritical system with carbon dioxide as the fluid. Three kinds of immobilized lipases such as Lipozyme RMIM, Novozyme 435 and Lipozyme TLIM were screened. The optimal esterification conditions for three kinds of immobilized lipases were achieved through single-factor tests. The results showed that the optimal preparation process parameters for three kinds of lipases were the addition amounts of 2.5%, 3% and 8% for Lipozyme RMIM, Novozyme 435 and Lipozyme TLIM, reaction temperatures of 65, 70 ℃ and 65 ℃ for three lipases, reaction time of 7, 8 h and 9 h for three lipases, and substrate ratio of 2:1. The yields of DG for three lipases were 68.6%, 67.7% and 64.8%, respectively, under their optimal reaction conditions. A two-step distillation technology was used to purify DG in the supercritical system. After the purification, the yield of DG was up to 60% and the purity of DG was improved to 90.4% from 68.6%. According to the comprehensive consideration, Lipozyme RMIM is recommended as lipase for the preparation of DG in industrial production.

Key words: CO2 supercritical system, lipase, glycerolysis, diacylglycerol, distillation

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