阴离子交换树脂分离酪蛋白糖巨肽工艺条件优化

doi:10.7506/spkx1002-6630-201202016

食品科学 ›› 2012, Vol. 33 ›› Issue (2): 72-77.doi: 10.7506/spkx1002-6630-201202016

阴离子交换树脂分离酪蛋白糖巨肽工艺条件优化

刁瑞丽，闫亚丽，陈庆森

天津商业大学生物技术与食品科学学院，天津市食品生物技术重点实验室

出版日期:2012-01-25 发布日期:2012-01-16
基金资助:
天津市科技计划项目(09ZCKFNC00700)；国家自然科学基金项目(30771524)

Optimization of Separation Process Conditions for Casein Glycomacropeptide by Anion Exchange Resin Adsorption

DIAO Rui-li，YAN Ya-li，CHEN Qing-sen

Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China

Online:2012-01-25 Published:2012-01-16

摘要/Abstract

摘要： 采用离子交换树脂从乳清粉中分离酪蛋白糖巨肽(casein glycomacropeptide，CGMP)，筛选适于分离CGMP的离子交换树脂并考察静态吸附过程中吸附pH值、吸附时间、缓冲液浓度等因素对CGMP分离效果的影响。乳清粉溶液在pH5.1时离心除杂后与201×4树脂混合，吸附条件为吸附pH3.9、吸附时间1h、缓冲液浓度0.02mol/L、洗脱液为0.5mol/L pH4.0的氯化钠溶液、洗脱时间4h；100g乳清粉利用此工艺条件可得1.45g唾液酸含量10.4%(以蛋白质计)的CGMP。该工艺方法分离过程简单、纯化效果好、唾液酸含量高，适用于工业化生产。

关键词: 酪蛋白糖巨肽（CGMP）, 阴离子交换树脂, 乳清粉, 唾液酸

Abstract: Casein glycomacropeptide (CGMP) is a kind of peptide that has many physiological functions and can be widely used in functional foods and medicine. Anion exchange resin has been used to separate casein glycomacropeptide from whey powder. The optimal separation conditions of anion exchange resin were investigated to explore the effects of adsorption pH, adsorption time and buffer concentration on separation efficiency of CGMP. The results showed that the appropriate exchange resin was resin 201×4. The optimal adsorption conditions were adsorption buffer pH of 5.1, adsorption time of 1 h, and adsorption buffer concentration of 0.02 mol/L. The optimal elution conditions were elution buffer concentration of 0.5 mol/L, elution time of 1 h and elution buffer pH of 4.0. After removing the impurity from whey powder at pH 5.1 by centrifugation, 1.45 g CGMP with sialic acid concentration of 10.4% was achieved from 100 g whey powder. The whole separation process conditions were simple and the separation efficiency was satisfory so that anion exchange resin is suitable for the industrial production of CGMP.

Key words: casein glycomacropeptide (CGMP), anion exchange resin, whey powder, sialic acid

中图分类号:

TS252.1

刁瑞丽, 闫亚丽, 陈庆森. 阴离子交换树脂分离酪蛋白糖巨肽工艺条件优化[J]. 食品科学, 2012, 33(2): 72-77.

DIAO Rui-li, YAN Ya-li, CHEN Qing-sen. Optimization of Separation Process Conditions for Casein Glycomacropeptide by Anion Exchange Resin Adsorption[J]. FOOD SCIENCE, 2012, 33(2): 72-77.

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阴离子交换树脂分离酪蛋白糖巨肽工艺条件优化

Optimization of Separation Process Conditions for Casein Glycomacropeptide by Anion Exchange Resin Adsorption

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