食品科学 ›› 2012, Vol. 33 ›› Issue (2): 139-142.doi: 10.7506/spkx1002-6630-201202030

• 工艺技术 • 上一篇    下一篇

响应面法优化大球盖菇粗多糖的提取工艺研究

陈君琛,赖谱富,周学划,翁敏劼,李怡彬   

  1. 福建省农业科学院农产品加工研究中心
  • 出版日期:2012-01-25 发布日期:2012-01-16
  • 基金资助:
    福建省农业科学院科技创新团队重点科研项目(CXTD2011-16);福建省公益类科研专项(2010R1017-4)

Optimization of Extraction Process for Crude Polysaccharides from Stropharia rugoso-annulata by Response Surface Methodology

CHEN Jun-chen,LAI Pu-fu,ZHOU Xue-hua,WENG Min-jie,LI Yi-bin   

  1. Agricultural Product Processing Research Centre, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
  • Online:2012-01-25 Published:2012-01-16

摘要: 采用响应面法优化大球盖菇粗多糖提取工艺。以提取温度、提取时间及料液比作为影响大球盖菇粗多糖得率的因素,通过单因素试验选取因素与水平,根据Box-Benhnken中心组合试验设计原理,在单因素试验的基础上采用三因素三水平响应面分析法,依据回归分析确定各工艺条件的主要影响因素。结果表明:大球盖菇粗多糖水浸提的最佳工艺条件为提取温度75℃、提取时间20min、料液比1:25(g/mL),大球盖菇粗多糖的得率达到7.614%。

关键词: 大球盖菇, 粗多糖, 响应面法

Abstract: The optimal extraction process for crude polysaccharides from stropharia rugoso-annulata was investigated by response surface methodology to explore the effects of extraction temperature, extraction time, extraction repeat number and solid-liquid ratio on the yield of crude polysaccharides. Based on the single-factor tests, Box-Behnken design with three key factors was conducted and a regression model was established. The results showed that optimal extraction process parameters were extraction temperature of 75 ℃, extraction time of 20 min and solid-liquid ratio of 1:25 (g/mL). Under the optimal extraction conditions, the yield of crude polysaccharides from Stropharia rugoso-annulata was up to 7.614%.

Key words: Stropharia rugoso-annulata, crude polysaccharides, response surface methodology (RSM)

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