食品科学 ›› 2012, Vol. 33 ›› Issue (2): 253-257.doi: 10.7506/spkx1002-6630-201202056

• 分析检测 • 上一篇    下一篇

原壳小球藻生物量快速测定方法的对比研究

崔 妍,李美芽,施春雷,史贤明   

  1. 上海交通大学农业与生物学院食品科学与工程系,中美食品安全联合研究中心,陆伯勋食品安全研究中心
  • 出版日期:2012-01-25 发布日期:2012-01-16
  • 基金资助:
    上海交通大学农业与生物学院新进博士教师基金项目(NQN200901);上海交通大学研究生创新能力培养专项基金项目

A Comparative Study of Rapid Determination Methods for Biomass of Chlorella protothecoides CS-41

CUI Yan,LI Mei-ya,SHI Chun-lei,SHI Xian-ming   

  1. MOST-USDA Joint Research Center for Food Safety, Bor Luh Food Safety Center, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Online:2012-01-25 Published:2012-01-16

摘要: 通过分光光度计和酶标仪分别测定不同浓度小球藻藻液的吸光度(A)和光密度(OD),用显微镜对细胞精确计数和用烘干法测量细胞干质量,分别在吸光度/光密度和细胞密度、吸光度/光密度和细胞干质量之间建立了良好的线性关系,相关系数较显著。经过一系列波长的筛选,确定在波长440nm处测定吸光度/光密度最佳,进而确定生物量。两种仪器对比显示:酶标仪测得的结果线性关系更加显著。与细胞计数法等传统方法相比,光密度法更加便捷,能极大地提高检测效率和准确度,可作为小球藻实验室研究和工业化生产的有效检测手段。

关键词: 原壳小球藻, 生物量, 吸光值, 显微镜计数, 干重

Abstract: Absorbance and optical density of Chlorella protothecoides CS-41 were measured by a spectrophotometer and a microplate spectrophotometer, respectively. Total cell number and dry biomass of C. protothecoides were evaluated by microscope and drying method. A good linear relationship between the absorbance/optical density and microscopic counting/biomass was established. The wavelength of 440 nm was chosen as the optimal wavelength for measuring absorbance/optical density of biomass. Through the comparison of both kinds of instruments, microplate spectrophotometer revealed more obvious linear relationship and optical density method could provide more convenience, efficiency and accuracy for the determination of biomass.

Key words: Chlorella protothecoides CS-4, biomass, absorbance, optical density, microscopic counting, dry weight

中图分类号: