关键词: 葛根, 香菇, 总黄酮, 共发酵

Abstract: Objective: To determine the effect of Lentinus edodes fermentation on the extraction efficiency of total flavonoids from Radix Puerariae and optimize culture medium ingredients and fermentation conditions. Methods: The optimization of medium ingredients and fermentation conditions was carried out using one-factor-at-a-time and orthogonal array design methods. Results: The optimal medium composition (g/100 mL) consisted of corn meal 2, peptone 0.2, monopotassium phosphate 0.5, and magnesium sulfate 0.2, and the optimal fermentation conditions were inoculum amount of 5%, Radix Puerariae concentration of 8 g/100 mL, fermentation temperature of 35℃, fermentation pH of 7, rotation speed of 150 r/min, and fermentation time of 144 h. Conclusions: Under these conditions, the content of total flavonoids was 2.727 mg/g, which revealed a 120.81% increase compared with the control group (without the inoculation of Lentinus edodes).

Key words: Radix Puerariae, Lentinus edodes, total flavonoids, fermentation