食品科学

• 营养卫生 • 上一篇    下一篇

细香葱提取物对人胃癌细胞SGC7901抑制作用

尤亚林,李 慧,潘思轶,徐晓云*   

  1. 华中农业大学食品科学技术学院,环境食品学教育部重点实验室,湖北 武汉 430070
  • 出版日期:2017-02-15 发布日期:2017-02-28

Inhibitory Effect of Chive Extract on the Proliferation of Human Gastric Cancer Cell Line SGC7901

YOU Yalin, LI Hui, PAN Siyi, XU Xiaoyun*   

  1. Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Online:2017-02-15 Published:2017-02-28

摘要: 通过四甲基偶氮唑蓝比色法和流式细胞术研究不同细香葱提取物对人胃癌细胞SGC7901增殖的抑制作用及其对细胞周期、凋亡率的影响。结果显示,细香葱抗胃癌活性成分主要集中在75%乙醇提取部位,同一区域不同品种细香葱抑制胃癌细胞增殖作用存在显著性差异(P<0.05),其中3号细香葱75%乙醇提取物的抑制作用最强,其24、48、72 h时IC50值分别为(0.88±0.07)、(0.45±0.08)、(0.21±0.03) mg/mL;SGC7901细胞经1.0、2.0 mg/mL的细香葱75%乙醇提取物处理48 h后,S期细胞所占比例显著增多(P<0.05),细香葱75%乙醇提取物阻碍人胃癌细胞SGC7901由S期向G2期的转化,将细胞周期阻滞于S期;同时,实验组细胞相比对照组凋亡率显著上升(P<0.05),存在量效关系。综上,细香葱75%乙醇提取物能够阻滞人胃癌细胞SGC7901由S期向G2期转化、促进其凋亡并抑制细胞增殖,细香葱具有开发成为抗胃癌功能食品的潜力。

关键词: 细香葱, 抗胃癌, 人胃癌细胞SGC7901

Abstract: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry were used to analyze the proliferation, cell cycle and apoptosis of human gastric cancer SGC7901 cells in the presence of chive extract. Results showed that 75% ethanol extract from chive (CEE) had the highest antitumor activity among different solvent extracts. There was a significant difference in the antitumor activities of chive extracts from different cultivars of the same geographic origin (P < 0.05). The 75% ethanol extract from chive cultivar No. 3 had the highest antitumor activity with IC50 values at 24, 48 and 72 h of (0.88 ± 0.07), (0.45 ± 0.08), and (0.21 ± 0.03) mg/mL, respectively. The proportion of S-phase cells significantly (P < 0.05) increased after treatment with this chive extract at 1.0 or 2.0 mg/mL for 48 h, indicating that CEE may inhibit cell transformation from S phase to G2 phase. CEE could also induce S phase arrest. Meanwhile, the apoptosis rate in the experimental group increased significantly (P < 0.05) in a dose-dependent manner when compared to the control group. Taken collectively, it is concluded that CEE is a promising natural anti-gastric cancer compound with potential applications in functional food industry for its effective inhibition on cell proliferation and cell cycle, and its promotion on cell apoptosis.

Key words: chive, anticancer activity, human gastric cancer SGC7901 cells

中图分类号: