食品科学

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ACE抑制肽构效关系及其酶法制备的研究进展

林 凯1,韩 雪1,张兰威1,2,*,谯 飞1,程大友1   

  1. 1.哈尔滨工业大学化工与化学学院,黑龙江 哈尔滨 150090;2.中国海洋大学食品科学与工程学院,山东 青岛 266003
  • 出版日期:2017-02-15 发布日期:2017-02-28

Progress in Structure-Activity Relationship and Enzymatic Preparation of ACE Inhibitory Peptides

LIN Kai1, HAN Xue1, ZHANG Lanwei1,2,*, QIAO Fei1, CHENG Dayou1   

  1. 1. School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150090, China;
    2. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
  • Online:2017-02-15 Published:2017-02-28

摘要: 高血压是一种全球性的公共健康问题。血管紧张素转换酶(angiotensin converting enzyme,ACE)作为一种二肽缩肽酶,通过肾素-血管紧张素系统和激肽释放酶-激肽系统(kallikrein-kinin system,KKS)对血压进行调节。近年来,已有许多从各种食源蛋白中获得ACE抑制肽的研究报道。相比于化学合成药物,食源性蛋白获得的ACE抑制肽在治疗高血压方面更具安全性和温和性。许多研究已经探讨了食源性降压肽的构效关系,尤其是多肽一级序列对活性的影响。目前,最常用于制备ACE抑制肽的方式是酶催化水解蛋白,包括使用单酶或复合酶对蛋白进行水解。本文将主要对ACE抑制肽构效关系和酶法制备ACE抑制肽的研究进展进行综述,以期为获得优良的ACE抑制肽产品提供理论指导。

关键词: 血管紧张素转换酶抑制肽, 降压机制, 构效关系, 酶法制备

Abstract: Hypertension is a considerable public health problem worldwide. Angiotensin-Ⅰ converting enzyme (dipeptidylcarboxypeptidase, EC 3.4.15.1, ACE) plays a critical role in regulating blood pressure through the renninangiotensin system (RAS) and kallikrein-kinnin system (KKS). Recently, numerous studies aimed at alleviating hypertension have focused on the generation and isolation of ACE-inhibitory peptides from various food sources. A number of studies have reported the structure-activity relationship of food protein-derived antihypertensive peptides, especially the effect of the primary structure on the potency. Compared with synthetic chemical drugs, ACE inhibitory peptides derived from food proteins are considered to be safer and milder. Nowadays, one of the most widely used techniques to liberate ACE inhibitory peptides is enzymatic hydrolysis. This technique involves one or more proteases at the optimum temperature and pH conditions. In this review, in order to provide a theoretical guidance for the preparation of high-quality ACE inhibitory peptides, we review recent reports on the structure-activity relationship and enzymatic preparation of ACE inhibitory peptides.

Key words: angiotensin-Ⅰ converting enzyme inhibitors, antihypertensive mechanism, structure-activity relationship, enzymatic preparation

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