食品科学 ›› 2017, Vol. 38 ›› Issue (4): 231-236.doi: 10.7506/spkx1002-6630-201704037

• 工艺技术 • 上一篇    下一篇

响应面试验优化黏质沙雷氏菌利用菜籽饼粕产灵菌红素工艺

陈新颜,操丽丽,杨培周,王康林,章 华,操新民,郑 志,姜绍通   

  1. 1.合肥工业大学食品科学与工程学院,安徽省农产品加工精深省级实验室,安徽 合肥 230009; 2.平光制药股份有限公司,安徽 合肥 231131
  • 出版日期:2017-02-25 发布日期:2017-02-28
  • 基金资助:
    合肥工业大学大学生创新创业训练项目(201510359056);安徽省科技攻关项目(1604a0702001)

Response Surface Optimization of Prodigiosin Production of Serratia marcescens Using Rapeseed Meal

CHEN Xinyan, CAO Lili, YANG Peizhou, WANG Kanglin, ZHANG Hua, CAO Xinmin, ZHENG Zhi, JIANG Shaotong   

  1. 1. Anhui Key Laboratory of Intensive Processing of Agricultural Products, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China; 2. Pingguang Pharmaceutical Limited by Share Ltd., Hefei 231131, China
  • Online:2017-02-25 Published:2017-02-28

摘要: 为筛选得到一株产灵菌红素的黏质沙雷氏菌为发酵菌种,采用响应面法优化培养基组分和发酵条件,提高黏质沙雷氏菌生产灵菌红素的效率,结果表明,黏质沙雷氏菌最优培养基组分及发酵条件为玉米粉用量10 g/L、菜籽饼粕用量21.7 g/L、硫酸锌质量浓度0.05 g/L、发酵液初始pH 5.8、接种量5.5%、装液量80 mL/250 mL三角瓶、温度27 ℃、摇床转速200 r/min,培养24 h后,发酵液中灵菌红素产量达到11.56 g/L。本研究为高温菜籽饼粕原料发酵生产灵菌红素提供理论依据。

关键词: 黏质沙雷氏菌, 灵菌红素, 菜籽饼粕, 响应面优化, 发酵

Abstract: A culture medium containing rapeseed meal was designed for prodigiosin production by Serratia marcescens. Optimizations of culture medium composition and fermentation conditions by response surface method were performed for improved production of prodigiosin. The results indicated that the optimal medium composition was composed of corn powder 10 g/L, rapeseed meal 21.7 g/L, inoculum concentration 5.5%, and zinc sulfate 0.05 g/L at an initial pH of 5.8. When the strain was cultured for 24 h in 80 mL of the medium contained in a 250 mL flask with shaking at 200 r/min, the fermented broth had the highest prodigiosin content of 11.56 g/L. This study could provide a basis for the microbial production of prodigiosin from high-temperature rapeseed meal.

Key words: Serratia marcescens, prodigiosin, rapeseed meal, response surface optimization, fermentation

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