桑沟湾养殖牡蛎中贝类毒素监测及预警方法的初步建立

摘要/Abstract

摘要： 研究了利用高效液相色谱-串联质谱（High Performance Chromatography Tandem Mass Spectrometry，LC-MS/MS）方法监测养殖牡蛎中五种腹泻性贝类毒素与六种麻痹性贝类毒素含量变化及分布特征，分别利用HP20大孔型吸附树脂与SP700吸附树脂作为富集树脂，富集养殖海域海水中五种腹泻性贝类毒素与六种麻痹性贝类毒素，利用LC-MS/MS检测方法分析其中毒素含量，同步监测了养殖海域内牡蛎与海水中毒素含量，探究了两者之间的关系，建立了牡蛎内贝类毒素含量随海水内贝类毒素含量之间的变化规律。实验结果显示：在该海域内一共监测到OA、DTX-1、GYM、PTX-2四种腹泻性贝类毒素与STX，dcSTX两种麻痹性贝类毒素；在整个监控期内，海水中所监测贝类毒素随时间变化呈现先增长，达到峰值后逐渐降低趋势。牡蛎中毒素含量与海水中毒素含量呈正相关关系，即牡蛎内毒素的增长虽海水内毒素的增长而增长，但牡蛎内毒素含量的峰值出现时间在海水中毒素含量出现峰值之后，延后时间约为两个采样周期。根据固相吸附毒素跟踪技术（Solid Phase Adsorbent Toxin Tracking，SPATT）技术原理，可以提前两个采样周期预警牡蛎内毒素含量。

关键词: 牡蛎, 树脂, 贝类毒素, 监测, 预警

Abstract: Monitoring the content change and distribution characteristics of five kinds of diarrhetic shellfish poisoning (DSP) and six kinds of paralytic shellfish poisoning (PSP) from oysters by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) were studied. Five kinds of DSP and six kinds of PSP from aquaculture waters were collected using HP20 adsorption resin and SP700 adsorption resin, respectively, and then the content was detected by LC-MS/MS. The oysters and seawater samples were collected and detected diarrheal toxin contents, synchronously then explored its variation characteristics and study variation disciplines between the oysters and seawater samples. The results showed that four kinds of DSP (OA、DTX-1、GYM、PTX-2) and two kinds of PSP (STX，dcSTX) within this area were detected from the oysters and seawater samples. During the monitoring period, shellfish toxins which wrere monitored in the seawater increased firstly as the time changed then gradually reduced after the peak. There is a positive correlation between the toxin content in oysters and in seawater, and the growth of oyster endotoxin increases with the increase of seawater endotoxin growth. It is surprisingly that the peak of oyster toxin content appeared after the peak of seawater toxin, the delay time is about two sampling period. According to the principle of SPATT technology, we can early warning toxin content in oyster two sampling period in advanced.

Key words: oysters, resins, shellfish toxins, monitoring, early warning

中图分类号:

TS207.7

宿志伟赵峰姜雪刘远平刘志敏牟伟丽周德庆. 桑沟湾养殖牡蛎中贝类毒素监测及预警方法的初步建立[J]. 食品科学, 0, (): 0-0.

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