关键词: 毛细管电泳, 电化学发光, 荷叶碱, 莲心碱

Abstract: A new method was developed for the determination of nuciferine in Nelumbinis Folium and liensinine in Nelumbinis Plumula by capillary electrophoresis coupled with electrochemilumolinescence (ECL) detection. The optimized experimental conditions were determined as follows: Ru(bpy)3 2+ concentration, 5 mmol/L; detection potential, 1.20 V; 10 mmol/L phosphate buffer (pH 5.74) as running buffer, 60 mmol/L (pH 8.30) phosphate buffer contained in the detection reservoir; injection time, 10 s; separation voltage, 13 kV. Detection limits (LOD) of 7.7 × 10-7 mol/L (RSN = 3) for nuciferine and 7.8 × 10-7 mol/L (RSN = 3) for liensinine were obtained. Relative standard derivations of electrophoretic peak area and migration time were 3.76% and 0.83% for 6.8 × 10-5 mol/L nuciferine and 4.28% and 1.37% for 3.1 × 10-5 mol/L liensinine from five replicate determinations, respectively. The method can be applied to detect nuciferine in lotus leaves and liensinine in Nelumbinis Plumula with satisfactory results.

Key words: capillary electrophoresis, electrochemiluminescence, nuciferine, liensinine