孕鼠补充苏氨酸锌对仔鼠生长及其相关基因表达的影响

doi:10.7506/spkx1002-6630-201705039

食品科学

孕鼠补充苏氨酸锌对仔鼠生长及其相关基因表达的影响

虞俊翔，冯建萍，谢明勇，王光然，周寰，胡晓波*

南昌大学食品科学与技术国家重点实验室，江西南昌 330047

出版日期:2017-03-15 发布日期:2017-03-28

Effect of Prepartum Supplementation of Pregnant Rats with Zinc Threoninate on the Growth Performance and Related Genes Expression of Their Offspring

YU Junxiang, FENG Jianping, XIE Mingyong, WANG Guangran, ZHOU Huan, HU Xiaobo*

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Online:2017-03-15 Published:2017-03-28

摘要/Abstract

摘要： 为研究母体补充苏氨酸锌对子代生长水平的影响，实验将40 只受孕SD大鼠随机分为5 组，于孕鼠妊娠期第6～15天分别灌胃补充高、中、低剂量苏氨酸锌（灌胃剂量分别为250、500、1 000 mg/（kg·d）），孕鼠分娩后将仔鼠常规饲养，并测量1 月龄仔鼠的生长指标和相关基因的表达。结果表明，苏氨酸锌各剂量组仔鼠体质量与对照组相比有显著提高（P＜0.05）；苏氨酸锌各剂量组与对照组相比较，其组织中锌转运体ZnT1基因的相对表达量都有显著提高（P＜0.05）；苏氨酸锌高剂量组与对照组相比较，其组织中胰岛素样生长因子IGF1基因相对表达量都有显著提高（P＜0.05）；且胰岛素样生长因子受体基因IGF1R的表达与IGF1的表达趋势基本保持一致。实验说明妊娠期补充苏氨酸锌能在一定程度上提升仔鼠的生长性能，能显著提高仔鼠锌转运体ZnT1、胰岛素样生长因子IGF1及其受体IGF1R基因表达水平。

关键词: 苏氨酸锌, 锌转运体ZnT1, 胰岛素样生长因子IGF1, 胰岛素样生长因子受体, 基因相对表达量

Abstract:

The purpose of this study was to observe the effect of prepartum supplementation of zinc threoninate (Thr-Zn) for pregnant rats on the growth performance of their offspring. Forty pregnant SD rats were randomly divided into five groups. Thr-Zn at high, medium and low doses during the sixth to fifteenth day of pregnancy was orally administered to the three experimental groups of animals, respectively. After the newborn rats were routinely fed to one month of age, the growth indicators and the expression of growth-related genes were evaluated. Compared with the control group, body weights of the offspring rats from each dose group increased significantly (P < 0.05). In addition, the mRNA expression levels of zinc transporter 1 (ZnT1) and insulin-like growth factor 1 (IGF1) were increased significantly (P < 0.05). The expression of insulin-like growth factor receptor (IGF1R) exhibited the same trend as IGF1. Therefore, the growth performance of offspring rats could be improved, and the mRNA expression levels of ZnT1, IGF1 and IGF1R could be increased significantly by prepartum zinc supplementation.

Key words: zinc threoninate, zinc transporter 1 (ZnT1), insulin-like growth factor 1 (IGF1), insulin-like growth factor receptor, relative gene expression level

中图分类号:

TS201.6

虞俊翔，冯建萍，谢明勇，王光然，周寰，胡晓波. 孕鼠补充苏氨酸锌对仔鼠生长及其相关基因表达的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201705039.

YU Junxiang, FENG Jianping, XIE Mingyong, WANG Guangran, ZHOU Huan, HU Xiaobo. Effect of Prepartum Supplementation of Pregnant Rats with Zinc Threoninate on the Growth Performance and Related Genes Expression of Their Offspring[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201705039.

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[2]	冯建萍，王光然，胡晓波，姜雯雯，谢明勇. 高剂量苏氨酸锌和硫酸锌对大鼠胚胎-胎仔发育毒性的比较[J]. 食品科学, 2014, 35(17): 241-244.
[3]	李川，朱科学，聂少平，胡晓波，黄丹菲，谢明勇. 苏氨酸锌对糖尿病大鼠肝脏损伤的保护作用[J]. 食品科学, 2011, 32(19): 198-200.
[4]	胡晓波1，龚毅1，郭智勇1,2，聂少平1，李昌1，王远兴1，谢明勇1,*. 葡聚糖凝胶过滤色谱柱法测定两种氨基酸锌螯合物螯合率的差异性[J]. 食品科学, 2009, 30(24 ): 397-400.

孕鼠补充苏氨酸锌对仔鼠生长及其相关基因表达的影响

Effect of Prepartum Supplementation of Pregnant Rats with Zinc Threoninate on the Growth Performance and Related Genes Expression of Their Offspring

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