食品科学 ›› 2011, Vol. 32 ›› Issue (4): 152-154.doi: 10.7506/spkx1002-6630-201104032

• 分析检测 • 上一篇    下一篇

凝胶渗透色谱-高效液相色谱法对辣椒酱中14种邻苯二甲酸酯的同时检测

张会军,张敬轩,李 挥,张 岩   

  1. 河北省食品质量监督检验研究院,河北省食品安全重点实验室
  • 出版日期:2011-03-12 发布日期:2017-04-06
  • 基金资助:
    河北省科技支撑计划项目(09227114D;09227134D)

Simultaneous Determination of 14 Phthalate esters in Chili Sauce by GPC-HPLC

ZHANG Hui-jun,ZHANG Jing-xuan,LI Hui,ZHANG Yan   

  1. Key Laboratory of Food Safety of Hebei, Hebei Institute of Food Quality Supervision Inspection and Research, Shijiazhuang 050051, China
  • Online:2011-03-12 Published:2017-04-06

摘要: 建立辣椒酱中14种邻苯二甲酸酯类环境激素的高效液相色谱-二极管阵列检测器(HPLC-DAD)检测方法。样品通过石油醚超声提取,GPC净化,氮吹浓缩后,以乙腈-水为流动相,经Warters-C18(250mm×4.6mm,5μm)液相色谱柱分离,进行HPLC-DAD分析。方法线性相关系数r>0.998,检出限为0.25~2.0mg/kg,在10mg/kg添加水平时样品加标回收率为85.7%~100.8%,RSD为0.37%~4.57%。该方法灵敏、快速、高通量,可用于14种邻苯二甲酸酯雌激素的测定。

关键词: 凝胶净化, 高效液相色谱, 辣椒酱, 邻苯二甲酸酯

Abstract: A GPC-HPLC-DAD method was established for the simultaneous determination of 14 kinds of phthalate esters (PAEs) in chili sauce. Samples were extracted with petroleum ether in an ultrasonic field, followed by clean-up by gel permeation chromatography and concentration by nitrogen gas blowing. The chromatographic separation was achieved on  Waters-C18 column (250 mm × 4.6 mm, 5μm) using an acetonitrile-water mobile phase. A HPLC system coupled with a diode array detector (DAD) was used to analyze the analytes. The developed analytical method exhibited a linear correlation coefficient of more than 0.998 and detection limits ranging from 0.25 to 2.0 mg/kg. Spike recovery rates between 85.7% and 100.8% were obtained, with relative standard deviations (RSD) of 0.37%-4.57%. Therefore, this method is a sensitive, rapid high throughout method and has good suitability to be used to analysis 14 kinds of PAEs in chili sauce.

Key words: gel permeation chromatography (GPC), high preformance liquid chromatography (HPLC), chili sauce, phthalate esters

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