高效液相色谱-串联质谱测定猪肝中阿维菌素、伊维菌素残留

doi:10.7506/spkx1002-6630-201104040

食品科学 ›› 2011, Vol. 32 ›› Issue (4): 185-188.doi: 10.7506/spkx1002-6630-201104040

高效液相色谱-串联质谱测定猪肝中阿维菌素、伊维菌素残留

郑卫东，胡江涛，阴文娅,，盛毅，武志雄

1.四川省产品质量监督检验检测院 2.四川出入境检验检疫局 3.四川大学华西公共卫生学院营养与食品卫生学教研室

出版日期:2011-03-12 发布日期:2017-04-06
基金资助:
四川省科技厅应用基础研究项目(04JY029-033)

Determination of Avermectin and Ivermectin Residues in Pork Liver by High Performance Liquid Chromatography-Mass Spectrometry

ZHENG Wei-dong，HU Jiang-tao，YIN Wen-ya，SHENG Yi，WU Zhi-xiong

1. Sichuan Institute of Product Quality Supervision and Inspection, Chengdu 610031, China； 2. Sichuan Entry-exit Inspection and Quarantine Bureau, Chengdu 610041, China； 3. Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China

Online:2011-03-12 Published:2017-04-06

摘要/Abstract

摘要： 目的：建立高效液相色谱-四极杆串联质谱仪(LC-MS/MS)同时测定猪肝脏中阿维菌素(AVM)和伊维菌素(IVM)的残留量。方法：猪肝样品均质后用乙腈提取，碱性氧化铝固相萃取柱(SPE)净化，采用串联质谱电喷雾电离ESI(+)，多反应监测模式(MRM)检测，以保留时间和子离子比定性，外标法定量。结果：方法检出限(LOD)均为2.0μg/kg，定量限(LOQ)为5.0μg/L，两种药物的线性范围均为5～100μg/L，相关系数均为0.9999。添加水平在2～20μg/kg范围内，阿维菌素和伊维菌素的平均回收率分别为77.0%～83.3%和76.9%～79.8%之间，相对标准偏差(RSD)分别小于12.1%和13.0%。结论：方法操作简单，灵敏准确，可满足当前国内外该产品中此类药物的检测要求。

关键词: 猪肝, 阿维菌素, 伊维菌素, 高效液相色谱-串联质谱

Abstract: Objective: To establish a method for the simultaneous determination of avermectin and ivermectin residues in pork liver using high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Methods: Avermectin and ivermectin residues were extracted with acetonitrile, cleaned up on a solid phase extraction (SPE) column containing alkaline aluminum oxide, separated on a C8 column, detected by tandem mass spectrometry with electrospray ionization and multiple reaction monitoring interface and quantitatively determined by high performance liquid chromatography in an external standard manner. Results: The limits of detection (LOD) for avermectin and ivermectin were 2μg/kg and limits of quantitation were 5μg/L. Both the linear ranges of avermectin and ivermectin were 5－100μg/L with correlation coefficients of both 0.9999. The recovery rate range of this method was 77.0%－83.3% for avermectin and 76.9%－79.8% for ivermectin with relative standard deviations (RSD) of 8.6%－12.1% and 9.9%－13.0% at spike levels of 2, 10μg/kg and 20μg/kg, respectively. Conclusion: This method is simple, sensitive and accurate, which can meet the domestic and international requirements for the detection of both veterinary drugs.

Key words: pork liver, avermectin, ivermectin, high performance liquid chromatography-tandem mass spectrometry

中图分类号:

R155.5

郑卫东，胡江涛，阴文娅,，盛毅，武志雄. 高效液相色谱-串联质谱测定猪肝中阿维菌素、伊维菌素残留[J]. 食品科学, 2011, 32(4): 185-188.

ZHENG Wei-dong，HU Jiang-tao，YIN Wen-ya，SHENG Yi，WU Zhi-xiong. Determination of Avermectin and Ivermectin Residues in Pork Liver by High Performance Liquid Chromatography-Mass Spectrometry[J]. FOOD SCIENCE, 2011, 32(4): 185-188.

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高效液相色谱-串联质谱测定猪肝中阿维菌素、伊维菌素残留

Determination of Avermectin and Ivermectin Residues in Pork Liver by High Performance Liquid Chromatography-Mass Spectrometry

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