明胶掺假乳制品快速检测方法的建立

doi:10.7506/spkx1002-6630-201104049

食品科学 ›› 2011, Vol. 32 ›› Issue (4): 220-222.doi: 10.7506/spkx1002-6630-201104049

明胶掺假乳制品快速检测方法的建立

魏战峰，田忙雀，王玥，杨水云

1.咸阳市疾病预防控制中心 2.西安市粮油质量检验中心 3.西安交通大学生命科学与技术学院

出版日期:2011-03-12 发布日期:2017-04-06

Development of a Rapid Detection Method for Glutin-adulterated Milk

WEI Zhan-feng，TIAN Mang-que，WANG Yue，YANG Shui-yun

1. Xianyang Center for Disease Control and Prevention, Xianyang 712000, China；2. Cereals and Oils Quality Test Center of Xi an, Xi an 710003, China；3. College of Life Science and Technology, Xi an Jiaotong University, Xi an 710069, China

Online:2011-03-12 Published:2017-04-06

摘要/Abstract

摘要： 目的：建立一种明胶掺假乳制品的快速定性方法。方法：采用硝酸汞溶液沉淀乳蛋白，进一步用苦味酸溶液检测明胶，通过敏感度测定试剂用量。结果：用1.4g/100mL硝酸汞溶液沉淀乳蛋白的最佳用量为乳品体积的1/2；用苦味酸检测明胶的敏感度为30mg/100mL。结论：该方法快速、方便，灵敏度高，可做成试剂盒，满足实际样品检测要求。

关键词: 掺假乳, 明胶检测, 试剂盒

Abstract: Objective: To develop a method for the rapid qualitification of glutin-adulterated milk. Methods: For the precipitation of milk proteins, Hg(NO3)2 solution was used, and the supernatant resulting from centrifugation was then added with saturated picric acid solution. After thorough mixing, whether or not the reaction system becomes turbid was identified by comparison with a negative control. The sensitivity of the established method was evaluated by using simulated adulterated milk samples with varying amounts of glutin. Meanwhile, Hg(NO3)2 solution amount and centrifugation conditions were optimized. Results: Addition of a half volume of 1.4 g/100 mL Hg(NO3)2 solution to milk samples provided optimum precipitation of milk proteins. The detection sensitivity of the method for glutin was 300 mg/100 mL. Conclusion: This method is rapid, convenient and highly sensitive, and has the potential to be developed into reagent kit products, which can meet the requirements for detection of real samples.

Key words: adulterated milk, glutin, reagent kit

中图分类号:

TS207.3

魏战峰，田忙雀，王玥，杨水云. 明胶掺假乳制品快速检测方法的建立[J]. 食品科学, 2011, 32(4): 220-222.

WEI Zhan-feng，TIAN Mang-que，WANG Yue，YANG Shui-yun. Development of a Rapid Detection Method for Glutin-adulterated Milk[J]. FOOD SCIENCE, 2011, 32(4): 220-222.

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明胶掺假乳制品快速检测方法的建立

Development of a Rapid Detection Method for Glutin-adulterated Milk

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