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• 生物工程 •    下一篇

红景天和轮叶党参混合提取物戊糖乳杆菌发酵条件及抗氧化作用研究

姜国哲1,全贞玉2,金润浩2,Hong Hee-doo3,韩春姬2   

  1. 1. 延边大学食品研究中心
    2. 延边大学医学院
    3. 韩国食品研究院
  • 收稿日期:2017-02-11 修回日期:2017-11-09 出版日期:2018-01-25 发布日期:2018-01-05
  • 通讯作者: 韩春姬 E-mail:chjihan@126.com
  • 基金资助:
    国家科技部国际合作专项基金项目

Fermentation Condition and Antioxidative Activity of Rhodiola sachalinensis and Codonopsis Lanceolata Mixture by Lactobacillus pentosus

  • Received:2017-02-11 Revised:2017-11-09 Online:2018-01-25 Published:2018-01-05
  • Contact: Chun-ji HAN E-mail:chjihan@126.com

摘要: 目的:优化红景天和轮叶党参混合提取物(RCME)戊糖乳杆菌发酵条件,分析发酵产物的主要活性成分组成与含量,评价其抗氧化作用。方法:红景天和轮叶党参混合提取物中接种戊糖乳杆菌,以发酵液中的pH值、总酸含量及生物量为指标,研究发酵所需的最佳时间、培养基组成(红景天和轮叶党参混合比例)、RCME最佳浓度;采用比色法测定RCME发酵前后的总蛋白质、中性糖、酸性多糖及总酚含量变化,采用高效液相色谱法分析RCME发酵前后的红景天苷和酪醇含量变化,采用DPPH和ABTS自由基清除实验评价RCME发酵物的抗氧化作用。结果:单纯红景天提取物发酵24~72h,其pH值和总酸含量无明显变化,随着发酵时间延长,乳杆菌数明显减少(P<0.01)。红景天和轮叶党参混合比例达到60%:40%,发酵24h后发酵液的pH下降幅度和总酸含量升高的幅度最大,发酵48h时后,pH值和总酸含量无明显的变化,乳杆菌数有所减少。可溶性固形物含量在1.0%~3.0%范围内,红景天苷转化为酪醇的转化率无明显差异。RCME发酵24h后,红景天苷含量显著减少(P<0.01),酸性多糖、总酚、蛋白质及酪醇含量均显著增加(P<0.05或P<0.01)。RCME发酵后DPPH和ABTS自由基清除能力明显增强。结论:红景天与轮叶党参混合提取物发酵条件为红景天-轮叶党参混合比例为60%:40%(w : w),可溶性固形物含量为1.0%~3.0%,发酵时间为24h。

关键词: 红景天, 轮叶党参, 戊糖乳杆菌, 发酵, 抗氧化

Abstract: Objective: To investigate optimization of fermentation condition and antioxidative activity of rhodiola sachalinensis-codonopsis lanceolata mixed extract (RCME) by lactobacillus pentosus. Method: Using pH value, acidity and viable cell counts as index, the optimum time, optimum mixture ratio of rhodiola sachalinensis(RS) and codonopsis lanceolate(CL) and RCME concentration were investigated. Total protein, neutral sugar, acidic polysaccharide and total phenolics content variation in RCME before and after fermentation was determined using colorimetric method. Antioxidant activity of fermented RCME using DPPH and ABTS radical scavenge experiment. Results: In the single fermentation of RS extract, the pH and total acidity rarely changed, and the viable cell counts decreased greatly(P<0.01) after fermented for 24~72 hours. When the mixing ratio of RS and CL was 60:40, the pH value was maximumly decreased, whereas the total acidity and viable cell counts maximumly increased. Soluble solid content of RCME was at range of 1.0%~3.0%, pH, viable cell counts and conversion rate of salidroside to tyrosol had no significant difference. In the fermentation product, Salidroside was significantly decreased (P<0.01), and tyrosol, acidic polysaccharide, total phenolics and protein were obviously increased (P<0.05 or P<0.01) after fermentation for 24 hours. DPPH radical and ABTS radical scavenge activity of fermentation product were significantly higher than that of RCME (P<0.01). Conclusion: Optimum fermentation condition of rhodiola sachalinensis-codonopsis lanceolata mixed extract are rhodiola sachalinensis-codonopsis lanceolata mixture at ratio of 60%:40%, soluble solid concentration of RCME at 1.0%~3% and fermented at 37℃ for 24 hours.

Key words: Rhodiola sachalinensis, Codonopsis lanceolata, Lactobacillus pentosus, fermentation, antioxidant activity

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