食品科学 ›› 2017, Vol. 38 ›› Issue (10): 178-182.doi: 10.7506/spkx1002-6630-201710030

• 成分分析 • 上一篇    下一篇

UPLC和Q-Exactive高分辨质谱仪联用鉴定 大黄鱼中的甘油三酯分子组成

翁丽萍,赵 芸,张 乐,王宏海   

  1. 1.杭州市农业科学研究院,浙江 杭州 310024;2.浙江工商大学环境科学与工程学院,浙江 杭州 310018
  • 出版日期:2017-05-25 发布日期:2017-05-23
  • 基金资助:
    国家自然科学基金青年科学基金项目(31301463)

Identification of Triacylglycerols in the Lipids of Pseudosciaena crocea by UPLC and Q-Exactive High-Resolution Mass Spectrometer

WENG Liping , ZHAO Yun , ZHANG Le , WANG Honghai   

  1. 1. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China; 2. School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, China
  • Online:2017-05-25 Published:2017-05-23

摘要: 应用超高效液相色谱和电喷雾/四极杆-静电场轨道阱的质谱联用技术,分析大黄鱼脂肪中甘油三酯的组 成。采用Hypersil Gold C18柱(150 mm×4.6 mm,2.1 μm),流动相为82%甲醇(含0.1%甲酸)溶液和18%异丙醇 溶液,混合后添加0.05%乙酸铵溶液,以等度洗脱方式分离大黄鱼脂肪中的甘油三酯组分,柱后流出液采用电喷雾 离子源,以正离子模式采集一级质谱分子离子和二级质谱的碎片离子信息,然后与Lipidblast数据库中甘油三酯信 息进行比对。研究结果显示,超高效液相色谱和Q-Exactive高分辨质谱仪联用技术快速、准确鉴定出大黄鱼脂质中 含有28 种甘油三酯。PoOL、PoSO、PPO、PPoO、PSO、MPO、POL、PoSA 8 种甘油三酯占总量的87.87%以上, 其中PoOL(C16:1、C18:1、C18:2)相对含量最丰富,占35.261%。

关键词: 大黄鱼, 脂质, 脂肪酸, 甘油三酯, 电喷雾离子源

Abstract: The triglyceride composition of Pseudosciaena crocea lipids was analyzed by ultra performance liquid chromatography-electrospray ionization/quadrupole/electrostatic field orbit trap high resolution mass spectrometry (UPLCESI/ Orbitrap-MS). The chromatographic separation was performed on a Hypersil Gold C18 column (150 mm × 4.6 mm, 2.1 μm) using a mobile phase consisting of methanol (including 0.1% formic acid)-isopropyl alcohol (82:18, V/V) and 0.05% ammonium acetate. The analytes were detected by ESI/Orbitrap-MS in the positive ion mode to obtain mass and tandem mass spectra together and then the results obtained were compared with those from the Lipidblast database. A total of 28 triacylglycerols were identified by UPLC-ESI/Orbitrap-MS). Among these, PoOL was the most abundant (35.261%), and PoOL, PoSO, PPO, PPoO, PSO, MPO, POL and PoSA together accounted for 87.87% of the total triacylglycerols.

Key words: Pseudosciaena crocea, lipid, fatty acid, triacylglycerols, electrospray ionization

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