食品科学 ›› 2017, Vol. 38 ›› Issue (10): 191-198.doi: 10.7506/spkx1002-6630-201710032

• 成分分析 • 上一篇    下一篇

不同胁迫温度条件下坛紫菜中脂肪酸和 挥发性物质分析

宋 悦,卓 馨,陈娟娟,骆其君,杨 锐,陈海敏,严小军   

  1. 宁波大学海洋学院,浙江 宁波 315211
  • 出版日期:2017-05-25 发布日期:2017-05-23
  • 基金资助:
    浙江省自然科学基金项目(LY17D060002);2015年度国家星火计划项目(2015GA701027); 浙江省科技厅公益项目(2016C37090);宁波大学王宽诚幸福基金资助项目

Analysis of Fatty Acids and Volatile Organic Compounds of Pyropia haitanensis under Heat Shock at Different Temperatures

SONG Yue, ZHUO Xin, CHEN Juanjuan , LUO Qijun, YANG Rui, CHEN Haimin, YAN Xiaojun   

  1. School of Marine Sciences, Ningbo University, Ningbo 315211, China
  • Online:2017-05-25 Published:2017-05-23

摘要: 为了解高温胁迫条件下坛紫菜脂肪酸变化规律,采用气相色谱-质谱联用技术对不同胁迫温度条件下的坛 紫菜脂肪酸和挥发性物质进行分析。结果显示,以20 ℃为对照温度,分别在25、28 ℃和35 ℃三个温度胁迫后,坛 紫菜中共鉴定出13 种总脂肪酸、14 种游离脂肪酸和32 种挥发性物质。当胁迫温度升高至28 ℃时,饱和脂肪酸和挥 发性物质含量随胁迫温度升高而增加,而不饱和脂肪酸和游离脂肪酸含量则随胁迫温度升高而降低;当胁迫温度升 高至35 ℃时,总脂肪酸、总游离不饱和脂肪酸和挥发性物质含量均随胁迫温度升高而降低。了解高温胁迫条件下 总脂肪酸、游离脂肪酸和挥发性物质之间的转化,有助于了解坛紫菜抗高温胁迫机理和坛紫菜的健康栽培。

关键词: 坛紫菜, 高温胁迫, 脂肪酸, 挥发性物质, 气相色谱-质谱法

Abstract: To understand the changes in the fatty acid profile of Pyropia haitanensis under heat shock stress, the fatty acids (FAs) and volatile organic compounds (VOCs) of P. haitanensis under heat shock stress at different temperatures were analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that after subjecting P. haitanensis to heat shock at 20 (control), 25, 28 and 35 ℃, a total of 13 total fatty acids (TFAs), 14 free fatty acids (FFAs) and 32 VOCs were identified. The contents of saturated fatty acids (SFAs) and VOCs were increased with increasing heat shock temperature up to 28 ℃, whereas the contents of unsaturated fatty acids (UFAs) and FFAs were decreased. As the heat shock temperature further rose to 35 ℃, the contents of TFAs, FFAs and VOCs were decreased. This study may be helpful to understand the mechanism of heat resistance of P. haitanensis and its cultivation.

Key words: Pyropia haitanensis, heat shock, fatty acid, volatile organic compounds, gas chromatography-mass spectrometry

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