花生油和玉米油中多组分真菌毒素高效
液相色谱-串联质谱检测方法的建立

doi:10.7506/spkx1002-6630-201710048

食品科学 ›› 2017, Vol. 38 ›› Issue (10): 297-304.doi: 10.7506/spkx1002-6630-201710048

花生油和玉米油中多组分真菌毒素高效液相色谱-串联质谱检测方法的建立

刘丹，韩小敏，李凤琴，王畅，罗晓林，卢大新,

1.北京农学院食品科学与工程学院，北京 102206；2.农产品有害微生物及农残安全检测与控制北京市重点实验室，北京 102206；3.国家食品安全风险评估中心，卫生部食品安全风险评估重点实验室，北京 100021； 4.中山大学公共卫生学院，广东广州 510080

出版日期:2017-05-25 发布日期:2017-05-23
基金资助:
国家自然科学基金青年科学基金项目（31301489）；北京市自然科学基金项目（7163235）

Development of High Performance Liquid Chromatography-Tandem Mass Spectrometry for Detection of Multi-Mycotoxins in Peanut Oil and Corn Oil

LIU Dan , HAN Xiaomin , LI Fengqin , WANG Chang , LUO Xiaolin , LU Daxin ,

1. College of Food Science and Engineering, Beijing University of Agriculture, Beijing 102206, China; 2. Beijing Key Laboratory of Agricultural Product Detection and Control for Spoilage Organisms and Pesticides, Beijing 102206, China; 3. Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Centre for Food Safety Risk Assessment, Beijing 100021, China; 4. School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China

Online:2017-05-25 Published:2017-05-23

摘要/Abstract

摘要： 建立花生油和玉米油中黄曲霉毒素、玉米赤霉烯酮、单端孢霉烯族化合物等13 种真菌毒素的高效液相色谱-串联质谱检测方法。样品经乙腈-水提取、MultiSep 226多功能净化柱净化后，分别用电喷雾正离子模式和负离子模式检测。经优化，正离子模式流动相为含2 mmol/L甲酸铵的0.1%甲酸-甲醇溶液，负离子模式流动相为 0.1%氨水-乙腈溶液。该方法对花生油和玉米油中13 种真菌毒素的检出限为0.05～3.00 μg/kg，定量限为0.10～ 10.00 μg/kg，平均加标回收率为66.6%～114.9%。所建方法操作简单、灵敏度高、重复性好，可用于花生油和玉米油中多组分真菌毒素的协同测定。

关键词: 真菌毒素, 花生油, 玉米油, 高效液相色谱-串联质谱

Abstract: A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the simultaneous detection of 13 mycotoxins including aflatoxins, zearalenone, and trichothecenes in peanut and corn oils. Samples were extracted with acetonitrile-water, followed by clean-up with a MultiSep 226 multifunctional column. The positive electrospray ionization mode (ESI+) and negative electrospray ionization mode (ESI-) were respectively employed to acquire signals. The mobile phase for ESI+ was composed of 0.1% formic acid containing 2 mmol/L ammonium formate (solvent A) and methanol (solvent B), while that for ESI- consisted of 0.1% ammonia water (solvent A) and acetonitrile (solvent B). The limits of detections (LODs) for 13 mycotoxins in peanut and corn oils ranged from 0.05 to 3.00 μg/kg and the limits of quantification (LOQ) ranged from 0.10 to 10.00 μg/kg. The mean recoveries ranged from 66.6% to 114.9%. The method developed in the present study had the advantages of simple operation, high sensitivity and good reproducibility, and so it could be used for the detection of multi-mycotoxins in peanut and corn oils.

Key words: mycotoxin, peanut oil, corn oil, high performance liquid chromatography-tandem mass spectrometry

中图分类号:

TS207.3

刘丹，韩小敏，李凤琴，王畅，罗晓林，卢大新,. 花生油和玉米油中多组分真菌毒素高效液相色谱-串联质谱检测方法的建立[J]. 食品科学, 2017, 38(10): 297-304.

LIU Dan , HAN Xiaomin , LI Fengqin , WANG Chang , LUO Xiaolin , LU Daxin ,. Development of High Performance Liquid Chromatography-Tandem Mass Spectrometry for Detection of Multi-Mycotoxins in Peanut Oil and Corn Oil[J]. FOOD SCIENCE, 2017, 38(10): 297-304.

[1]	宁亚维，刘茁，杨正，马俊美，范素芳，李强，张岩. UPLC-MS/MS法同时检测食品中3种主要牛奶过敏原[J]. 食品科学, 2021, 42(8): 270-276.
[2]	刘文静，黄彪，傅建炜，韦航，黄财标. 超高效液相色谱-串联质谱法同时测定陈年老茶中16 种真菌毒素残留[J]. 食品科学, 2021, 42(2): 299-305.
[3]	戴尽波，沈洁，何啸峰，聂荣荣，董文静，梁沁娴. QuEChERS-超高效液相色谱-串联质谱法检测禽源性食品中氟虫腈及其代谢物[J]. 食品科学, 2021, 42(2): 325-332.
[4]	龚蕾，黄徽，韩智，江丰，彭青枝，黄宗骞，王亨. QuEChERS-UPLC-MS/MS测定果蔬中18 种琥珀酸脱氢酶抑制剂类杀菌剂[J]. 食品科学, 2021, 42(18): 261-268.
[5]	陈瑞鹏，孙云凤，霍冰洋，秦英凯，李双，梁俊，周焕英，高志贤. 真菌毒素多重检测技术研究进展[J]. 食品科学, 2021, 42(17): 267-274.
[6]	王可利，叶泰，徐斐，曹慧，袁敏，于劲松，阴凤琴，吴秀秀. UPLC-MS/MS法测定黄酒中8 种生物胺[J]. 食品科学, 2021, 42(16): 281-285.
[7]	张娜娜，陈利容，张守梅，郭玉秋，刘开昌，龚魁杰. 鲜食玉米籽粒发育期间污染真菌分离鉴定及真菌毒素含量分析[J]. 食品科学, 2021, 42(16): 273-280.
[8]	刘慧，吴颖，黄华. 脱氧雪腐镰刀菌烯醇及其衍生物检测方法的研究进展[J]. 食品科学, 2021, 42(15): 356-365.
[9]	曲劲尧，林煜程，毛馨，卢国柱，袁智鹏，张瑶瑶，尤艳莉，李彦伸. 链格孢霉菌侵染番茄产毒机制[J]. 食品科学, 2021, 42(15): 180-186.
[10]	马丽莎，谢文平，尹怡，单奇，刘书贵，李丽春，赵成，郑光明. QuEChERS-高效液相色谱-串联质谱法测定稻田水产品中氟虫腈及其代谢物残留[J]. 食品科学, 2021, 42(14): 308-314.
[11]	廖惠云，吴洋，马梦婕，毛淑蕊，朱怀远，陈晶波，张华，曹毅，朱龙杰，袁益来. 基质匹配曲线校正-HPLC-MS/MS法同时测定干性食品包装纸中8 种甜味剂[J]. 食品科学, 2021, 42(12): 328-333.
[12]	颜伟华，周莹，郭浩炜，王笑笑. UPLC-MS/MS快速筛查豆芽中27 种植物生长调节剂和抗生素类药物[J]. 食品科学, 2021, 42(12): 302-308.
[13]	刘志恬，董玉鹏，李永才，毕阳，李宝军，景春苑. ε-聚赖氨酸对梨果实黑斑病菌Alternaria alternata的抑制作用及其机理[J]. 食品科学, 2021, 42(11): 213-220.
[14]	徐洪文，徐华，朱瑜，叶永丽，孙嘉笛，孙秀兰，张晓娟. 磁性固相萃取技术在食品真菌毒素分析中的应用[J]. 食品科学, 2021, 42(11): 261-270.
[15]	张艳侠，赵慧男，孙珊珊，徐向军，郑文静，薛霞，王明栋，刘艳明，祝建华. 通过式SPE-UPLC-MS/MS测定植物油中的9 种酚类抗氧化剂[J]. 食品科学, 2021, 42(10): 322-329.

花生油和玉米油中多组分真菌毒素高效液相色谱-串联质谱检测方法的建立

Development of High Performance Liquid Chromatography-Tandem Mass Spectrometry for Detection of Multi-Mycotoxins in Peanut Oil and Corn Oil

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

花生油和玉米油中多组分真菌毒素高效 液相色谱-串联质谱检测方法的建立

Development of High Performance Liquid Chromatography-Tandem Mass Spectrometry for Detection of Multi-Mycotoxins in Peanut Oil and Corn Oil

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

花生油和玉米油中多组分真菌毒素高效液相色谱-串联质谱检测方法的建立