食品科学 ›› 2017, Vol. 38 ›› Issue (11): 168-173.doi: 10.7506/spkx1002-6630-201711027

• 基础研究 • 上一篇    下一篇

龙胆多糖的结构表征及其体外免疫活性

曹荣安,徐秀丽,苗家尉,郭增旺,李良玉,贾 建,王长远   

  1. 1.黑龙江八一农垦大学食品学院,黑龙江 大庆 163319;2.黑龙江八一农垦大学动物科技学院,黑龙江 大庆 163319;3.农业部农产加工品质量监督检验测试中心(大庆),黑龙江 大庆 163319;4.大庆市食品药品检验检测中心,黑龙江 大庆 163311;5.黑龙江八一农垦大学 国家杂粮工程技术研究中心,黑龙江 大庆 163319
  • 出版日期:2017-06-15 发布日期:2017-06-19

Structural Characterization and in Vitro Immunomodulatory Activity of Polysaccharides from Gentiana scabra Bunge

CAO Rong’an, XU Xiuli, MIAO Jiawei, GUO Zengwang, LI Liangyu, JIA Jian, WANG Changyuan   

  1. 1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 3. Postdoctoral Research Station in Agricultural Products Processing Quality Supervision, Inspection and Testing Center (Daqing), Ministry of Agriculture, Daqing 163319, China; 4. Daqing Center for Food and Drug Control, Daqing 163311, China; 5. National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • Online:2017-06-15 Published:2017-06-19

摘要: 对龙胆多糖的结构和生物活性进行研究,采用甲基化分析多糖的链接方式。结果表明,龙胆多糖中存在(→1)末端和(1→5)-链接的呋喃阿拉伯糖基、(1→4)-链接的吡喃半乳糖基,同时也存在其他含量比较少的糖基和分支。龙胆多糖中糖醛酸是以(1→4)-链接的半乳糖醛酸方式存在,对龙胆多糖的生物活性起着重要的作用。龙胆多糖可以促进RAW264.7细胞增殖并产生NO,而且NO的产生是通过一氧化氮合成酶mRNA上调实现的,同时龙胆多糖可以上调细胞因子COX-2和TNF-α的mRNA表达水平。

关键词: 龙胆, 多糖, 结构, 免疫活性

Abstract: As an extension of our previous work, where polysaccharides were extracted and purified from the rhizomes and roots of Gentiana scabra Bunge, this study was undertaken to investigate the structure and bioactivity of the polysaccharides. The type of glycosidic linkage was elucidated by methylation analysis. The backbones of the polysaccharides consisted of (→1) terminal and (1→5)-linked arabinofuranosyl, and (1→4)-linked galactopyranosyl residues with small amounts of other residues and branches. The uronic acid contained in the polysaccharides was composed of (1→4)-linked galacturonic acid residues, which played an important role in the bioactivity of the polysaccharides. The polysaccharides could enhance the proliferation of RAW264.7 cells to produce NO due to the increased mRNA expression of NO synthetase. The polysaccharides also could upregulate the mRNA expression of the cytokines COX-2 and TNF-α.

Key words: Gentiana scabra Bunge, polysaccharide, structure, immunomodulatory activity

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