食品科学 ›› 2017, Vol. 38 ›› Issue (12): 50-56.doi: 10.7506/spkx1002-6630-201712008

• 生物工程 • 上一篇    下一篇

野生嗜杀白假丝酵母LFA418的产毒条件优化及其毒素粗提物特性

李丽,冯莉,秦义,叶冬青,宋育阳,刘延琳   

  1. 1.西北农林科技大学葡萄酒学院,陕西?杨凌 712100;2.陕西省葡萄与葡萄酒工程技术研究中心,陕西?杨凌 712100
  • 出版日期:2017-06-25 发布日期:2017-06-26
  • 基金资助:
    国家现代农业(葡萄)产业技术体系建设专项(CARS-30-jg-3);国家自然科学基金面上项目(31571812)

Optimization of Culture Conditions for Toxin Production by Wild Killer Yeast Candida albicans LFA418

LI Li, FENG Li, QIN Yi, YE Dongqing, SONG Yuyang, LIU Yanlin,   

  1. 1. College of Enology, Northwest A&F University, Yangling 712100, China; 2. Shaanxi Engineering Research Center for Wine and Viticulture, Yangling 712100, China
  • Online:2017-06-25 Published:2017-06-26

摘要: 对1?株分离自新疆楼兰葡萄酒厂野生白假丝酵母(Candida albicans)LFA418的嗜杀特征及其粗毒素特性进行研究。结果显示,C. albicans LFA418具有致死克鲁维毕赤酵母(Pichia kluyveri)、贝酵母(Saccharomyces bayanus)、Lachancea thermotolerans和葡萄牙假丝酵母(Candida lusitaniae)的嗜杀性。接种体积分数7% C. albicans LFA418到YEPD液体培养基中,25?℃培养32?h,可以获得最大产量的嗜杀毒素;利用截留分子质量为8?000D的透析袋制备的粗毒素,在温度分别-20、4?℃?时pH?4.2~4.6和pH?4.2时温度4~20?℃具有嗜杀活性的稳定性。

关键词: 嗜杀酵母, 嗜杀毒素, 白假丝酵母

Abstract: The killer property Candida albicans LFA418, a wild strain isolated from a winery in Loulan of Northwest China’sXinjiang region. The killer feature and crude killer toxin characteristics of a wild yeast strain Candida albicans isolated from Loulan winery of Xinjiang were studied. The results indicated that Candida albicans LFA418 had a wide spectrum of killering activity against Pichia kluyveri, Saccharomyces bayanus, Lachancea thermotolerans and Candida lusitaniae. The optimal culture conditions to obtain the maximum yield of killer toxins were as follows: inoculums of C. albicans LFA418, 7% (V/V); temperature, 25 ℃, and culture time, 32 h. The activity of crude killer toxins was stable at pH 4.2-4.6 when temperature was -20 ℃ and 4 ℃ and temperature 4-20 ℃ at pH 4.2.

Key words: killer yeast, killer toxin, Candida albicans

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