食品科学 ›› 2017, Vol. 38 ›› Issue (12): 63-68.doi: 10.7506/spkx1002-6630-201712010

• 生物工程 • 上一篇    下一篇

高糖胁迫下槲皮素对酿酒酵母胞内损伤的保护作用与机制

徐晓丹,潘宇,柯尊丽,周志钦   

  1. 1.西南大学园艺园林学院,重庆 400716;2.中国农业科学院柑桔研究所,农业部柑桔产品质量安全风险评估实验室重庆,重庆 400715
  • 出版日期:2017-06-25 发布日期:2017-06-26
  • 基金资助:
    重庆市基础与前沿研究计划项目(cstc2014jcyjA80026); 2016食用农产品特征性营养组成识别与验证评估专项项目(GJFP201601501;GJFP201601503);重庆市现代特色效益农业技术体系创新项目(20164-4)

Protective Effect and Mechanism of Quercetin against High Glucose-Induced Intracellular Damage in Saccharomyces cerevisiae

XU Xiaodan, PAN Yu, KE Zunli, ZHOU Zhiqin,   

  1. 1. College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China; 2. Laboratory of Quality & Safety Risk Assessment for Citrus Products Chongqing, Ministry of Agriculture, Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400715, China
  • Online:2017-06-25 Published:2017-06-26

摘要: 以酿酒酵母S288c为模型,分析高糖胁迫下槲皮素对其胞内损伤的保护作用及机制。结果表明:与对照相比,高糖胁迫不影响酵母胞内活性氧(reactive oxygen species,ROS)水平,但显著降低了胞内酶比活力(P<0.05);槲皮素处理后,与对照组和高糖组相比,酿酒酵母胞内ROS水平、超氧化物歧化酶和过氧化氢酶活力均显著下降(P<0.05),而过氧化物酶(peroxidase,POD)比活力极显著升高(P<0.01),说明POD比活力对高糖耐受性反应更为灵敏,可作为衡量高糖胁迫应激机制的重要生理指标,槲皮素可通过调节胞内POD比活力来提高机体的抗氧化能力。另外,实时荧光定量聚合酶链式反应结果表明高质量浓度葡萄糖显著抑制了酵母中GPD2和SUC2的表达水平(P<0.05),并极显著提高了HXT1的表达水平(P<0.01),而对GUT1的表达影响不显著;槲皮素处理后,高糖胁迫下酵母中GPD2、SUC2和HXT1的表达水平显著提高(P<0.05),而GUT1无显著变化,说明槲皮素可能通过高渗透甘油途径、菊糖水解途径和己糖转运途径等来促进葡萄糖的分解代谢,从而达到保护机体细胞免受伤害的作用。结果表明槲皮素对高糖诱导的酿酒酵母胞内损伤具有保护作用,其作用机制可能与自身的抗氧化作用以及利用调节机体内高渗透甘油途径与糖的分解和转运途径存在一定的关联性。

关键词: 酿酒酵母, 高糖胁迫, 槲皮素, 酶比活力, 基因表达水平

Abstract: In this study, the protective effect of quercetin on high glucose-induced damage was investigated in Saccharomyces cerevisiae. Compared with the control group, the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were all significantly decreased under high glucose-induced stress (P < 0.05), but intracellular ROS was no obviously changed. After being treated with quercetin, the ROS levels and SOD and CAT activities were dramatically inhibited (P < 0.05), but the POD activity was significantly enhanced in comparison to the control and high glucose groups (P < 0.01). The results showed that POD activity was more sensitive than other indicators in reflecting the resistance to high glucose-induced stress, and quercetin could effectively enhance the antioxidant capacity by regulating POD activity in S. cerevisiae S288c. In addition, high glucose-induced stress resulted in an significant decrease in the expression levels of glycerol-3-phosphate dehydrogenase 2 (GPD2) and sucrose transport protein-encoding genes (SUC2) (P < 0.05), but a significant increase in the expression level of hexose transport-1-encoding gene (HXT1) (P < 0.01), compared with the control. After being treated with quercetin, the expression profiles of GPD2, SUC2, and HXT1 were significantly increased in S. cerevisiae S288c (P < 0.05). However, the expression level of glycerol kinase gene (GUT1) was not changed significantly. These results suggested that quercetin could reduce high glucose-induced damage by regulating the high osmolarity glycerol (HOG) signaling pathway, inulin catabolism pathway and hexose transport pathway to accelerate glucose decomposition. Therefore, we conclude that quercetin plays an important role in protecting S. cerevisiae from damage caused by high glucose-induced stress, and the underlying mechanism may be associated with its antioxidant effect and being involved in the HOG signaling pathway and the glucose decomposition and transport pathways in S. cerevisiae.

Key words: Saccharomyces cerevisiae, high glucose-induced stress, quercetin, enzyme activitity, expression levels

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