食品科学 ›› 2017, Vol. 38 ›› Issue (12): 151-156.doi: 10.7506/spkx1002-6630-201712023

• 成分分析 • 上一篇    下一篇

溶剂法提取分蘖葱头有机硫化合物及其GC-MS分析

刘婷婷,秦宇婷,吴玉莹,张晶,张艳荣   

  1. 吉林农业大学食品科学与工程学院,吉林?长春 130118
  • 出版日期:2017-06-25 发布日期:2017-06-26
  • 基金资助:
    林农业大学人才计划项目(20160509)

Solvent Extraction and GC-MS Analysis of Organic Sulfur-Containing Compounds from Tillering Onion

LIU Tingting, QIN Yuting, WU Yuying, ZHANG Jing, ZHANG Yanrong   

  1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
  • Online:2017-06-25 Published:2017-06-26

摘要: 为研究有机溶剂对分蘖葱头中有机硫化合物提取及成分的影响,采用二氯甲烷、乙酸乙酯、正己烷3?种溶剂提取分蘖葱头中有机硫化合物,并利用顶空固相微萃取和气相色谱-质谱联用技术对不同溶剂提取到有机硫化合物进行比较分析。结果表明:二氯甲烷提取分蘖葱头中有机硫化合物的最佳工艺条件为浸提时间4?h、料液比1∶4(g/mL)、搅拌速率250?r/min,在此条件下有机硫化合物得率为(0.413?3±0.007)%。乙酸乙酯、正己烷有机硫化合物得率分别为(0.273?3±0.011)%、(0.288?9±0.009)%。3?种溶剂共提取分离出45?种组分,均检测出9?种有机硫化合物,其中相对含量较高的均为噻吩类和硫醚类。另外,以二氯甲烷为溶剂提取出独有的有机硫化合物12?种;以乙酸乙酯为溶剂提取出独有的有机硫化合物3?种;以正己烷为溶剂提取出独有的有机硫化合物8?种。

关键词: 有机溶剂提取, 分蘖葱头, 有机硫化合物, 气相色谱-质谱联用

Abstract: In this study, we examined the effects of different organic solvents on the extraction efficiency and composition of organic sulfur-containing compounds from tillering onion. The organic sulfur-containing compounds were extracted by dichloromethane and ethyl acetate and n-hexane, respectively, and then analyzed by headspace solid phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC-MS). The optimum extraction conditions with dichloromethane as the extraction solvent were determined to be 4 h extraction at a ratio of material to liquid of 1:4 (g/mL) with stirring at 250 r/min, leading to a yield of (0.413 3 ± 0.007)%, which was higher than those obtained with ethyl acetate and n-hexane as the extraction solvent, (0.273 3 ± 0.011)% and (0.288 9 ± 0.009)%, respectively. A total of 45 components were extracted by the three solvents and 9 organic sulfur-containing compounds were detected in each solvent extract, among which, thiophenes and thioethers were predominant. In addition, 12, 3 and 8 unique organic sulfur-containing compounds were extracted by methylene chloride, ethyl acetate, and n-hexane.

Key words: organic solvent extraction, tillering onion, organic sulfur-containing compounds, gas chromatography-mass spectrometry

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