离子液体双水相萃取-HPLC分析红酒中的痕量氯酚类物质

doi:10.7506/spkx1002-6630-201712040

食品科学 ›› 2017, Vol. 38 ›› Issue (12): 260-265.doi: 10.7506/spkx1002-6630-201712040

离子液体双水相萃取-HPLC分析红酒中的痕量氯酚类物质

刘曼，施敏，曹学丽

北京工商大学食品学院，北京食品营养与人类健康高精尖创新中心，北京 100048

出版日期:2017-06-25 发布日期:2017-06-26
基金资助:
北京市自然科学基金重点项目（KZ201410011016）

Determination of Trace Chlorophenols in Red Wine by Ionic Liquid Aqueous Two-Phase Extraction Coupled to HPLC

LIU Man, SHI Min, CAO Xueli

Beijing Advanced Innovation Center for Food Nutrition and Human Health, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China

Online:2017-06-25 Published:2017-06-26

摘要/Abstract

摘要： 建立亲水性离子液体[C4MIM]BF4与无机盐(NH4)2SO4形成的离子液体双水相萃取富集红酒样品中6?种痕量氯酚类物质的方法。通过考察离子液体和盐的种类、pH值、离子液体和盐质量分数对氯酚萃取率和富集倍数的影响，确定萃取氯酚的最优条件。在最佳萃取条件下，6?种氯酚的线性范围为20～200?μg/L，相关系数（R2）达到0.999，6?种氯酚的检出限在3.68～12.16?μg/L之间。对实际红酒样品进行测定，加标回收率为87.73%～103.44%，相对标准偏差在0.33%～6.35%之间。该法操作简单、迅速、绿色且具有较高的萃取效率。

关键词: 离子液体, 双水相, 氯酚, 红酒, 高效液相色谱

Abstract: An extraction method based on an aqueous two-phase system consisting of the hydrophilic ionic liquid [C4MIM]BF4 and the inorganic salt (NH4)2SO4 for the simultaneous determination of six chlorophenols in red wine by high performance liquid chromatography (HPLC) was established. The extraction efficiency and enrichment factor were found to be influenced by the types and concentrations of salts and ionic liquids, as well as pH value, and these parameters were investigated to determine the optimal conditions for extracting chlorophenols. Under the optimal conditions, the linear ranges of six chlorophenols were 20–200 μg/L with correlation coefficients (R2) of 0.999, and the limits of detection (LODs) were in the range of 3.68–12.16 μg/L. This method was successfully applied to analyze six chlorophenols in real red wine samples with spiked recoveries ranging from 87.73% to 103.44% and relative standard deviation (RSD) ranging from 0.33% to 6.35%. This method is simple, rapid, environmentally friendly and efficient.

Key words: ionic liquid, aqueous two-phase system, chlorophenols, red wine, high performance liquid chromatography (HPLC)

中图分类号:

TS262.6

刘曼，施敏，曹学丽. 离子液体双水相萃取-HPLC分析红酒中的痕量氯酚类物质[J]. 食品科学, 2017, 38(12): 260-265.

LIU Man, SHI Min, CAO Xueli. Determination of Trace Chlorophenols in Red Wine by Ionic Liquid Aqueous Two-Phase Extraction Coupled to HPLC[J]. FOOD SCIENCE, 2017, 38(12): 260-265.

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离子液体双水相萃取-HPLC分析红酒中的痕量氯酚类物质

Determination of Trace Chlorophenols in Red Wine by Ionic Liquid Aqueous Two-Phase Extraction Coupled to HPLC

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