文冠果落果黄酮及抑菌性研究

摘要/Abstract

摘要： 目的：提取并测定文冠果落果黄酮的含量、单体及抑菌活性。方法：采用紫外分光光度法测定黄酮含量，采用显色反应、傅利叶红外光谱和高效液相色谱三者相结合的方法检测分析黄酮单体，同时测定文冠果落果黄酮对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌的抑菌活性。结果：文冠果落果中黄酮含量为18.4 mg/g；含有芦丁单体及其他14种未知的黄酮类单体物质，同时测得文冠果落果黄酮中芦丁的含量为27 mg/g，占文冠果落果总黄酮的6%左右；文冠果落果黄酮对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌均有一定的抑制作用，其中对大肠杆菌抑制效果最佳；结论：文冠果落果中黄酮含量丰富，含有抗氧化活性成分芦丁以及良好的抑菌活性。

关键词: 文冠果落果, 黄酮, 高效液相色谱, 红外光谱, 抑菌性

Abstract: Objective: Extraction and determination of content and monomer and antibacterial activity of flavonoids from Xanthoceras sorbifolia Bunge fruit drop. Methods: The contents of flavonoids was determined spectrometrically, color reaction and infrared spectrum analysis and high performance liquid chromatography (HPLC) analysis were used to analysis the flavonoids monomer composition of Xanthoceras sorbifolia Bunge fruit drop, and antibacterial activity of Bacillus subtilis and Escherichia coli and Staphylococcus aureus. Results: The content of flavonoids was 18.4 mg/g; There were rutin and other 14 kinds of unknown flavonoids monomer substance in Xanthoceras sorbifolia Bunge fruit drop, simultaneously determined the content of rutin was 27 mg/g in flavonoids from Xanthoceras sorbifolia Bunge fruit drop, which was about 6% of flavonoids of Xanthoceras sorbifolia Bunge fruit drop; Flavonoids of Xanthoceras sorbifolia Bunge fruit drop had inhibitory effect for Bacillus subtilis and Escherichia coli and Staphylococcus aureus, in which the best inhibiting effects on Escherichia coli. Conclusion: Abundant flavonoid in Xanthoceras sorbifolia Bunge fruit drop, which has antioxidant active ingredient rutin and excellent antibacterial activity.

Key words: Xanthoceras sorbifolia Bunge fruit drop, flavonoids, high performance liquid chromatography (HPLC), infrared spectrum, antibacterial activity

中图分类号:

TS202.1

杨小慧石光波拜晓彬赵晨煊李博生. 文冠果落果黄酮及抑菌性研究[J]. 食品科学, 0, (): 0-0.

Xiao-hui Yang Bo-sheng LI. Flavonoids and Its Antibacterial Activity of Xanthoceras sorbifolia Bunge Fruit Drop[J]. FOOD SCIENCE, 0, (): 0-0.

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