食品科学 ›› 2017, Vol. 38 ›› Issue (14): 175-180.doi: 10.7506/spkx1002-6630-201714027

• 成分分析 • 上一篇    下一篇

鸡肉酶解液制备鸡汤热反应过程中呈味物质的变化

侯佰慧,夏杨毅,,周涛,张维悦   

  1. (1.西南大学食品科学学院,重庆 400715;2.重庆市特色食品工程技术研究中心,重庆 400715)
  • 出版日期:2017-07-25 发布日期:2017-09-06
  • 基金资助:
    四川省科技支撑计划项目(12ZC2439);公益性行业(农业)科研专项(201303082-7)

Change of Taste Components in Thermal Reaction Model System Containing Chicken Enzymatic Hydrolysate with Reaction Time

HOU Baihui, XIA Yangyi, ZHOU Tao, ZHANG Weiyue   

  1. (1. College of Food Science, Southwest University, Chongqing 400715, China;2. Chongqing Special Food Programme and Technology Research Center, Chongqing 400715, China)
  • Online:2017-07-25 Published:2017-09-06

摘要: 以鸡胸肉、木瓜蛋白酶和葡萄糖为原料建立热反应体系,借助气相色谱-质谱联用仪和全自动氨基酸分析仪,研究热反应过程鸡肉酶解液呈味物质的变化。结果表明:随着加热时间延长游离氨基酸和呈味氨基酸含量均显著增加(P<0.05),其中苦味氨基酸占主导。实验中共检测出66?种挥发性化合物,对照组中检测出37?种,热反应组分别检测出44、51、52?种,包括醛类25?种、烷烃类7?种、酮类6?种、醇类8?种、酸类13?种、杂环类1?种、芳香类2?种、酯类3?种、烯烃类1?种;其中共有成分24?种。挥发性化合物含量随热反应时间延长而增加,90?min时达到最大值,即鸡肉酶解液在热反应条件下更易产生挥发性化合物。

关键词: 酶解液, 鸡汤, 热反应, 氨基酸, 风味

Abstract: In this study, a thermal reaction model system consisting of papain hydrolysate of chicken breast and glucose was built and used to investigate the change of taste components during the reaction process through analysis by?gas chromatography-mass spectrometry (GC-MS) and using an automatic amino acid analyzer. The?results?indicated?that with the increase in heating time, the contents of free amino acids and taste-active amino acids increased significantly (P < 0.05), with bitter amino acids being the dominant ones. A total of 66 volatile compounds including 25 aldehydes, 7 alkanes, 6 ketones, 8 alchols, 13 acids, 1 heterocyclic compound, 2 aromatics, 3 esters and 1 alkene compound were detected, 37 of which were detected in the control group (not heated), and 44, 51 and 52 of which were detected at different reaction times of 30, 60 and 90 min, respectively. Twenty-four volatile compounds were common to these four samples. The content of volatile compounds increased with increasing reaction time, reaching a maximum at 90 min. In conclusion, volatile compounds could be more easily produced chicken enzymatic hydrolysate under thermal reaction conditions.

Key words: enzymatic hydrolysate, chicken soup, thermal reaction, amino acids, volatile flavor compounds

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