食品科学 ›› 2017, Vol. 38 ›› Issue (13): 60-65.doi: 10.7506/spkx1002-6630-201713010

• 基础研究 • 上一篇    下一篇

脂质体在婴儿体外胃肠道消化的膜结构稳定性

冯炎雯,李 娜,徐纪璇,邬琰泽,韩剑众,刘玮琳   

  1. 浙江工商大学食品与生物工程学院,浙江 杭州 310018
  • 出版日期:2017-07-15 发布日期:2017-07-11

Membrane Stability of Liposomes during in Vitro Simulated Infant Gastrointestinal Digestion

FENG Yanwen, LI Na, XU Jixuan, WU Yanze, HAN Jianzhong, LIU Weilin   

  1. School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China
  • Online:2017-07-15 Published:2017-07-11

摘要: 建立体外模拟婴儿胃肠道消化体系(成人为对照),研究脂质体在胃部的氧化应激效应和在小肠环境的脂解动力学。结果表明:脂质体消化后平均粒径明显减小、Zeta电位负电性增加;脂质体经胃部消化磷脂膜氧化程度(硫代巴比妥酸值)明显提高;在小肠消化过程中不断释放游离脂肪酸,且婴儿胃肠道环境水解率(38%)明显低于成人(80%),释放过程符合伪一级方程;傅里叶变换红外光谱研究发现,磷脂特征官能团(C=O、P=O、—CH2)峰值发生改变,脂质体在婴儿胃肠道环境膜结构破坏程度低于成人。研究结果为脂质体运载营养素应用于婴儿配方食品提供了理论指导。

关键词: 脂质体, 体外消化, 婴儿, 膜结构, 稳定性

Abstract: In order to study the effect of liposomes on gastric oxidative stress and the lipolysis kinetics of liposomes during gastric and small intestinal digestion, an in vitro infant gastrointestinal system was established, with its adult counterpart as control. Results showed that the average diameter of liposomes was significantly decreased and the negative zeta potential was increased after digestion. The oxidation degree of liposomal membrane, which was characterized by thiobarbituric acid reactive substances (TBARS), significantly increased after gastric digestion. Free fatty acids were released continuously during stimulated intestinal digestion, and the rate of lipolysis in the infant gastrointestinal system (38%) was obviously higher than that in the adult one (80%). The release profiles fitted first-order kinetics. Moreover, Fourier transform infrared spectroscopy (FT-IR) showed that the peaks of characteristic groups in phospholipids (C=O, P=O and ?CH2) changed after digestion, and the liposomal membrane in the infant gastrointestinal system was damaged less seriously than in the adult one. This study can provide a theoretical guidance for liposomes as a nutrient carrier in infant formula.

Key words: liposome, in vitro digestion, infant, membrane structure, stability

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