食品科学 ›› 2017, Vol. 38 ›› Issue (13): 182-189.doi: 10.7506/spkx1002-6630-201713030

• 营养卫生 • 上一篇    下一篇

尿石素A对巨噬细胞极化及巨噬-泡沫细胞形成的作用

韩淇安,刘红燕,闫春红,石 超,夏效东   

  1. 1.西北农林科技大学食品科学与工程学院,陕西 杨凌 712100;2.中国农业大学食品科学与营养工程学院,北京食品营养与人类健康高精尖创新中心,北京 100083;3.定兴县医院,河北 定兴 072650;4.西安交通大学生命科学与技术学院,陕西 西安 710049
  • 出版日期:2017-07-15 发布日期:2017-07-11

Effect of Urolithin A on Macrophage Polarization and the Formation of Macrophage-Derived Foam Cells

HAN Qi’an, LIU Hongyan, YAN Chunhong, SHI Chao, XIA Xiaodong   

  1. 1. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China; 2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University,Beijing 100083, China; 3. Dingxing Hospital, Dingxing 072650, China; 4. School of Life Science and Technology,Xi’an Jiaotong University, Xi’an 710049, China
  • Online:2017-07-15 Published:2017-07-11

摘要: 旨在探究尿石素A对巨噬细胞极化及对巨噬-泡沫细胞形成的影响及相关的分子机制。结果发现,尿石素A通过调控不同标志基因的表达,不仅能够抑制RAW264.7小鼠巨噬细胞向促炎的M1型巨噬细胞极化,还能促进自然状态巨噬细胞向M2型巨噬细胞极化;油红O染色发现尿石素A能够显著抑制巨噬-泡沫细胞形成,实时荧光定量聚合酶链式反应检测说明尿石素A能够抑制胆固醇合成基因羟甲基戊二酸单酰辅酶A还原酶和脂肪酸合成酶基因的转录;此外,尿石素A显著上调三磷酸腺苷结合盒转运蛋白A1和G1基因的表达,该两个基因表达与促进了胆固醇的排出相关。本研究证明了尿石素A对巨噬细胞极化具有调控作用,同时尿石素A能够抑制巨噬-泡沫细胞形成和胆固醇合成。这些结果揭示了尿石素A具有潜在的抗动脉粥样硬化的作用,为之后深入研究提供了理论参考。

关键词: 尿石素A, 巨噬细胞极化, 泡沫细胞, 胆固醇, 动脉粥样硬化

Abstract: This research was designed to investigate the potential anti-atherogenic effect of urolithin A on macrophage polarization and the formation of macrophage derived-foam cells as well as the underlying molecular mechanisms. It was found that urolithin A could mediate the mRNA expression of different marker genes in M1-type and natural macrophages, indicating its ability to inhibit the polarization of macrophage cell line (RAW264.7) into pro-inflammatory M1-type macrophage and to promote the polarization of macrophage cells into M2 type. The results of oil red O staining revealed that urolithin A inhibited the formation of foam cells derived from macrophages. Real time PCR showed that urolithin A also markedly decreased the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase and fatty acid synthase at the transcriptional level. Besides, urolithin A was capable of up-regulating the mRNA expression of the ATP-binding cassette transporters A1 and G1, which was positively associated with macrophage cholesterol efflux. In summary, the findings confirmed the essential role urolithin A plays in macrophage polarization, and further demonstrated the potential molecular mechanisms of urolithin A in preventing foam cell formation and cholesterol synthesis, which are of great significance in investigating and understanding the anti-atherogenic effect of urolithin A in the future.

Key words: urolithin A (Uro-A), macrophage polarization, foam cells, cholesterol, atherosclerosis

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