食品科学 ›› 2017, Vol. 38 ›› Issue (13): 190-195.doi: 10.7506/spkx1002-6630-201713031

• 营养卫生 • 上一篇    下一篇

文蛤寡肽对小鼠急性肝损伤的保护作用

王佳佳,赵莎莎,杨最素,余方苗,丁国芳   

  1. 浙江海洋大学食品与医药学院,浙江省海洋生物医用制品工程技术研究中心,浙江 舟山 316022
  • 出版日期:2017-07-15 发布日期:2017-07-11

Protective Effect of Meretrix meretrix Oligopeptides on Acute Liver Injury in Mice

WANG Jiajia, ZHAO Shasha, YANG Zuisu, YU Fangmiao, DING Guofang   

  1. Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Medical, Zhejiang Ocean University, Zhoushan 316022, China
  • Online:2017-07-15 Published:2017-07-11

摘要: 对文蛤寡肽(Meretrix meretrix oligopeptides,MMO)在小鼠急性肝损伤中的保护作用进行研究。首先通过酶解、超滤、Sephadex G-25柱分离技术从文蛤中分离纯化出具有肝修复作用的文蛤寡肽,经检测该目标肽的氨基酸序列为Gln-Leu-Asn-Trp-Asp。然后建立四氯化碳(CCl4)致小鼠急性肝损伤模型,测定小鼠肝脏指数;检测小鼠血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、γ-谷氨酰转移酶(γ-glutamyltransferase,γ-GT)、超氧化物歧化酶(superoxide dismutase,SOD)活力及甘油三酯(triglyceride,TG)、丙二醛(malondialdehyde,MDA)水平;苏木精-伊红染色观察肝脏病理学改变;免疫组化法测定肝组织TNF-α、NF-κB蛋白表达量。结果表明:MMO组与模型组比较,小鼠肝指数显著降低(P<0.05);血清中ALT、AST、γ-GT活力显著降低(P<0.05),γ-GT活力最高降幅达到47.16%;SOD活力显著升高(P<0.05);TG、MDA水平显著降低(P<0.05),最高降幅分别达到31.88%和28.83%。;苏木精-伊红染色观察肝组织结构明显好转;TNF-α、NF-κB蛋白表达量显著降低。因此,MMO对CCl4小鼠急性肝损伤有较为明显的保护作用。

关键词: 文蛤寡肽, 四氯化碳, 急性肝损伤

Abstract: The protective effect of Meretrix meretrix oligopeptide (MMO) on CCl4-induced acute liver injury in mice was examined. MMO, with the potential to repair damaged liver, was enzymatically extracted and purified by ultrafiltration and Sephadex G-25 column chromatography, and its amino acid sequence was determined to be Gln-Leu-Asn-Trp-Asp. Then, an acute liver injury model was established by intraperitoneal injection of CCl4 into mice. The liver index of mice was measured, the activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyltransferase (γ-GT) and superoxide dismutase (SOD) were determined, and serum triglyceride (TG) and malondialdehyde (MDA) levels were measured. Liver tissue samples from mice were stained with hematoxylin-eosin (HE) to observe the effect of MMO on liver histopathology. Immunohistochemical staining was used to detect the protein expression of TNF-α and NF-κB in hepatic tissue. The results showed that the MMO group exhibited a decrease in liver index, a reduction in serum ALT, AST and γ-GT activities (by up to 47.16%), an elevation in serum SOD activity, and a decline in serum TG and MDA levels (by up to 31.88% and 28.83%) compared with the model group, all these changes being statistically significant. Mouse liver tissue stained with HE revealed a notable improvement in liver histology. The protein expression of TNF-α and NF-κB in hepatic tissue was significantly decreased by administration of MMO. Therefore, MMO can effectively protect against acute liver injury induced by CCl4 in mice.

Key words: Meretrix meretrix oligopeptides, carbon tetrachloride, acute liver injury

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