关键词: 食源性副溶血性弧菌, 免疫磁珠分离, 酶联免疫吸附测定, 免疫层析测试, 免疫传感器, 免疫荧光显微术

Abstract: The bacterial pathogen Vibrio parahaemolyticus (VP) can pollute a variety of foods, especially seafoods. Foodborne VP has surged as the primary pathogen in food safety incidents in China. Compared to traditional cultural methods and modern molecular techhniques, specific antigen-antibody recognition-based immunological identification and subtyping strategies exhibit superiority in efficiency, specificity, on-field applicability and simplicity of pre-treatment. In this paper, the category and preparation methods of anti-VP antibodies at present are outlined. Furthermore, we review the current applications of immunological methods such as immunomagnetic separation (IMS), enzyme-linked immunosorbent assay (ELISA), immunochromatographic assay (ICA), immunosensor and immunomicroscopy in the detection of foodborne VP, which will provide useful references for rapid detection of VP.

Key words: foodborne Vibrio parahaemolyticus, immunomagnetic separation (IMS), enzyme-linked immunosorbent assay (ELISA), immunochromatographic assay (ICA), immunosensor, immunofluorescence microscopy (IFM)