食品科学 ›› 2012, Vol. 33 ›› Issue (5): 177-182.doi: 10.7506/spkx1002-6630-201205038

• 生物工程 • 上一篇    下一篇

浓香型白酒窖泥中高产己酸兼性厌氧细菌的分离鉴定

赵 辉,敞 颜,王 葳,凌宏志,平文祥,赵志昌,杨朝辉   

  1. 1.黑龙江大学生命科学学院 2.农业微生物技术教育部工程研究中心 3.黑龙江省富裕老窖酒业有限公司
  • 出版日期:2012-03-15 发布日期:2012-03-03
  • 基金资助:
    哈尔滨市科技局科技创新人才专项基金项目(2011RFQXN056);黑龙江省教育厅科学技术研究项目(12511409)

Isolation and Identification of Facultative Anaerobic Strains with High Yield of Hexanoic Acid from Luzhou-flavor Liquor Pit Mud

ZHAO Hui,CHANG Yan,WANG Wei,LING Hong-zhi,PING Wen-xiang,ZHAO Zhi-chang,YANG Zhao-hui   

  1. 1. College of Life Science, Heilongjiang University, Harbin 150080, China; 2. Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Harbin 150500, China; 3. Heilongjiang Fuyulaojiao Brewery Co. Ltd., Fuyu 161200, China
  • Online:2012-03-15 Published:2012-03-03

摘要: 筛选及鉴定白酒窖泥中高产己酸的细菌,以应用于窖池保养及人工窖泥培养。从东北地区某酒厂优质窖泥中采用兼性厌氧培养及微量成分分析的方法,分离筛选出3株(C78、a57、A17)高产己酸的兼性厌氧细菌,其产己酸量分别为:213.6914、170.465、103.5097mg/100mL,经形态学、生理生化特征和分子生物学鉴定其分别为:巨大芽孢杆菌(Bacillus megaterium)、梭状芽孢杆菌(Bacillus fusiformis)及地衣芽孢杆菌(Bacillus licheniformis),相应最适pH值分别为7、6.5、7,最适温度分别为34、34、37℃,最适接种量分别为5%、5%、3%。

关键词: 浓香型白酒, 窖泥, 产己酸细菌, 鉴定

Abstract: In order to screen and identify hexanoic acid-producing anaerobic bacteria with high yield from liquor pit mud and to apply it in maintaining pits or artificial pit mud. The hexanoic acid-producing anaerobic strains with high yield were isolated and selected from the fine pit mud of the distillery in the northeast area by facultative anaerobic culture and trace component analysis. The yields of hexanoic acid were up to 213.6914, 170.465 mg/100 mL and 103.5097 mg/100 mL, respectively. The species identification showed that three strains were Bacillus fusiformis, Bacillus licheniformis and Bacillus megaterium, respectively, through morphological observation, physiological and biochemical tests and molecular biology methods. The optimal pH was 7, 6.5 and 7 and the optimal temperatures were 34, 34 ℃ and 37 ℃ as well as the optimal inoculums were 5%, 5% and 3%. This study provides a theatrical guidance for the application of efficiently artificial pit mud.

Key words: Luzhou-flavor liquor, pit mud, hexanoic acid-producing bacteria, identification

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