响应曲面法优化酶解条件制备乳源ACE抑制肽

doi:10.7506/spkx1002-6630-201205044

食品科学 ›› 2012, Vol. 33 ›› Issue (5): 208-212.doi: 10.7506/spkx1002-6630-201205044

响应曲面法优化酶解条件制备乳源ACE抑制肽

徐鑫，蔡丽丽，刘国艳，何佳易，魏晓蕊，王之颖，张军

1.扬州大学食品科学与工程学院 2. 扬州大学实验农牧场

出版日期:2012-03-15 发布日期:2012-03-03
基金资助:
江苏省高校自然科学基础研究项目(08KJD550004)；扬州市-扬州大学合作基金项目(YZ2008089)；江苏省科技型中小企业技术创新资金项目(BN2008207)；江苏省普通高校研究生科研创新计划项目(CXLX11_1017)

Response Surface Methodology for Optimization of Hydrolysis Conditions for the Production of Milk-derived Angiotensin-I Converting Enzyme (ACE) Inhibitory Peptides

XU Xin，CAI Li-li，LIU Guo-yan，HE Jia-yi，WEI Xiao-rui，Wang Zhi-ying，ZHANG Jun

1. School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China； 2. Farm of Experiment, Yangzhou University, Yangzhou 225001, China

Online:2012-03-15 Published:2012-03-03

摘要/Abstract

摘要： 采用响应曲面法优化胰蛋白酶(PTN6.0S)酶解酪蛋白酸钠的工艺条件，制备高活性的血管紧张素转换酶(ACE)抑制肽。利用准确度更高的RP-HPLC法测定酶解产物的ACE抑制率，通过单因素和响应面试验设计，分别考察pH值、温度、时间、底物质量浓度、酶与底物比等因素对ACE抑制肽活性的影响。结果显示：响应曲面法优化酶解条件得到数学模型为：抑制率/%＝－11.21347＋4.32902A－1.45953B＋3.42928C－0.20303D＋0.050303AB＋0.047422AD＋0.14955BC＋0.12486BD－0.054526A2－0.079754B2－0.53587C2－0.28096D2，确定最佳工艺条件为pH7.0、温度52.31℃、时间19.44h、底物质量浓度5.91g/100mL、酶与底物比8.37‰，此时ACE抑制率达97.11%。

关键词: 酪蛋白酸钠, 胰蛋白酶, ACE抑制肽, 响应曲面法, RP-HPLC

Abstract: In our present study, response surface methodology was used to optimize process conditions for the hydrolysis of sodium caseinate by trypsin to prepare highly active angiotensin-I converting enzyme (ACE) inhibitory peptides. ACE inhibitory rate was measured by RP-HPLC. One-factor-at-a-time method followed by response surface analysis was used to analyze the effects of pH, temperature, hydrolysis time, substrate concentration, enzyme/substrate ratio on ACE inhibitory activity of sodium caseinate hydrolysate. A mathematical model describing the relationship of ACE inhibitory activity of sodium caseinate hydrolysate with temperature (A), hydrolysis time (B), substrate concentration (C) and enzyme/substrate ratio (D) was obtained as follows: Y=－11.21347＋4.32902A－1.45953B＋3.42928C－0.20303D＋0.050303AB＋0.047422AD＋0.14955BC ＋0.12486BD － 0.054526A2 － 0.079754B2 － 0.53587C2 － 0.28096D2. The optimum hydrolysis conditions were determined as pH 7.0, 52.31 ℃, 19.44 h, substrate concentration of 5.91 g/100 mL and enzyme/substrate ratio of 8.37‰, resulting in an ACE inhibitory rate of 97.11%.

Key words: sodium caseinate, trypsin, ACE inhibiting peptides, response surface methodology, RP-HPLC

中图分类号:

TS218

徐鑫，蔡丽丽，刘国艳，何佳易，魏晓蕊，王之颖，张军. 响应曲面法优化酶解条件制备乳源ACE抑制肽[J]. 食品科学, 2012, 33(5): 208-212.

XU Xin，CAI Li-li，LIU Guo-yan，HE Jia-yi，WEI Xiao-rui，Wang Zhi-ying，ZHANG Jun. Response Surface Methodology for Optimization of Hydrolysis Conditions for the Production of Milk-derived Angiotensin-I Converting Enzyme (ACE) Inhibitory Peptides[J]. FOOD SCIENCE, 2012, 33(5): 208-212.

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响应曲面法优化酶解条件制备乳源ACE抑制肽

Response Surface Methodology for Optimization of Hydrolysis Conditions for the Production of Milk-derived Angiotensin-I Converting Enzyme (ACE) Inhibitory Peptides

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