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• 成分分析 •    下一篇

高效液相色谱串联三重四级杆飞行时间质谱法鉴别五味子中木脂素成分

丁博,陈文锐,王志元,谢建军,曾广丰,王璐   

  1. 广东出入境检验检疫局检验检疫技术中心
  • 收稿日期:2017-03-29 修回日期:2018-01-15 出版日期:2018-04-25 发布日期:2018-04-17
  • 通讯作者: 丁博 E-mail:dingbo@mail2.sysu.edu.cn
  • 基金资助:
    保健食品中功效成分及违禁西药成分检测技术研究;基于高分辨质谱和同位素质谱的咖啡因类饮品天然成分真假鉴定技术研究

Identification of lignanoids in Schisandra chinensis by high performance liquid chromatography coupled with quadrupole time of flight mass spectrometry

2, 2, 2, 2, 2   

  • Received:2017-03-29 Revised:2018-01-15 Online:2018-04-25 Published:2018-04-17

摘要: 建立高效液相色谱串联三重四级杆飞行时间质谱法(HPLC-Q-TOF-MS)结合QuEChERS前处理方法对五味子中木脂素成分的快速筛查测定。样品经乙腈提取,优化QuEChERS净化条件,采用Xbridge BEH C18色谱柱分离,梯度洗脱,IDA-MS高分辨质谱检测模式,依据目标物的一级母离子的精确质量数、同位素丰度比二级离子的精确质量数和色谱保留时间等信息定性定量分析五味子中木脂素成分。结果表明:QuEChERS与LC-Q-TOF-MS分析方法快速筛查五味子中木脂素成分的选择性好,灵敏度高,准确性高;将该方法用于5个五味子样品的鉴定,明显筛查鉴别出南五味子和北五味子。

关键词: 五味子, 高效液相色谱串联三重四级杆飞行时间质谱法, 木脂素, QuEChERS

Abstract: A high performance liquid chromatography-quadrupole-time of flight mass spectrometry (LC-Q-TOF-MS) compared with QuEChERS pretreatment analytical method was established for quick screening lignanoids in Schisandra chinensis. Samples were extracted by acetonitrile, cleaned up by QuEChERS, and separated by Xbridge BEH C18 LC column. They were eluted with gradient solvent and carried out using information dependent acquisition high resolution mass spectrometry (IDA-MS). The qualitative and quantitative analysis was based on the accurate masses of the first level of parent molecular ions and isotopic patterns, and accurate masses of second level of fragment ions and a priori known retention times. The result showed that QuEChERS coupled with LC-Q-TOF-MS was effective for the identification of lignanoids in Schisandra chinensis with high selectivity, high sensitivity and high precision. The method was successfully applied to identify five Schisandra chinensis samples, Schisandra sphenanthera Rehd. et Wils and Schisandra chinensis(Turcz) Baill of which were significant characterization.

Key words: Schisandra chinensis, LC-Q-TOF-MS, lignanoids, QuEChERS

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