Fe3+修饰磁性纳米粒子的制备表征及对卵黄高磷蛋白吸附作用研究

摘要/Abstract

摘要： 通过化学共沉法和表面功能化修饰得到硫酸软骨素钠和铁离子负载的磁性纳米粒子Fe3O4-CS@Fe3+(简写为CMNP@Fe3+)，并对该粒子的形貌等特性进行分析。透射电镜观察显示CMNP@Fe3+呈尺寸为20 nm的圆球形，分散性较好；磁滞回线结果表明该粒子具有超顺磁性；红外光谱测定证明硫酸软骨素钠和Fe3+已成功修饰在Fe3O4表面。利用磁性纳米粒子表面Fe3+与卵黄高磷蛋白的强结合力，建立从蛋黄中磁性分离卵黄高磷蛋白的新方法，并对吸附过程的影响因素进行研究。结果发现当溶液pH为4.0，底物初始浓度为10 mg/mL，吸附时间为180 min时，磁性纳米粒子的吸附能力最强。利用动力学模型和等温吸附模型进行拟合，确定CMNP@Fe3+吸附卵黄高磷蛋白的过程符合伪二级动力学模型和Freundlich等温吸附模型，并通过模型计算得到吸附平衡时卵黄高磷蛋白的理论吸附量为625.00 mg/g。该研究为鸡蛋中蛋白质实现磁性分离提供依据。

关键词: 卵黄高磷蛋白, 磁性纳米粒子, 磁性分离, 硫酸软骨素钠, 铁离子

Abstract: Fuctional magnetic nanopaticles as Fe3O4-CS@Fe3+ (short as CMNP@Fe3+) were prepared by chemical co-precipitating and functional modification with sodium chondroitin sulfate and ferric ion, and their surface profile were analyzed. CMNP@Fe3+ showed spherical shapes with small diameter about 20 nm, and well idspersed in aqueous solution, which was proved by transmission electron microscope. It was found that the nanoparticles have superparamagnetic properties by hysteresis loop. Fourier infrared spectrum revealed sodium chondroitin sulfate and Fe3+ have been coated the surface of Fe3O4 successfully. This research built a new method to extract phosvitin from hen egg yolk used magnetic nanoparticles that based on phsovitin has strong bonding force with ferric ion, and investigated the influence factors of the adsorption process. Magnetic nanopartices has maximum adsorption capacity when the condition as pH was 4.0 of solution, initial substrate concentration was 10 mg/mL, and adsorption time was 180 min. The adsoption kinetics between CMNP@Fe3+ and phosvitin was better described by pseudo-second-order kinetic model, then the theoretical adsorption capacity was 625.00 mg/g when the adsorption equilibrium, and the data pointed well fit to Freundlich isotherm model .This research provide a reference for protein separation from eggs.

Key words: phosvitin, magnetic nanopaticles, magnetic separation, sodium chondroitin sulfate, ferric ion

中图分类号:

TS253.1

陈婵黄茜李珊珊马美湖. Fe3+修饰磁性纳米粒子的制备表征及对卵黄高磷蛋白吸附作用研究[J]. 食品科学, 0, (): 0-0.

Huang Xi Shan shanLi Ma Meihu. Synthesis and Characterization of Fe3+ Modified Magnetic Nanopaticles for Phosvitin Adsorption[J]. FOOD SCIENCE, 0, (): 0-0.

参考文献

[1]程晓燕, 吴子健, 王亮, 等.卵黄高磷蛋白及其磷酸肽的研究进展[J].食品科学, 2013, 34(21):43-47 [2]张晓维.卵黄高磷蛋白的分离纯化、结构表征及功能特性研究[D]. 武汉: 华中农业大学. 2014: 3-20. [3]REN J, WU J.Preparation of high purity egg phosvitin using anion exchange chromatography[J].[J]., 2014, 158:186-191- [4]ZHANG X W, QIU N, Geng F, et al.Simply and effectively preparing high-purity phosvitin using polyethylene glycol and anion-exchange chromatography[J]. Journal of separation science, 2011, 34: 3295-3301. DOI: 10.1002/jssc.201100601. [5]JUNG S, AHN D U, NAM K C, et al.Separation of phosvitin from egg yolk without using organic solvents[J]. Asian-Australasian journal of animal sciences, 2013, 26: 1622. DOI: 10.5713/ajas.2013.13263. [6]REN J, WU J.Thermal-aided phosvitin extraction from egg yolk[J].Journal of the Science of Food and Agriculture, 2015, 95(13):2595-2600 [7]TING C P, BERTRAND P, POULIOT Y, et al.On the use of ultrafiltration for the concentration and desalting of phosvitin from egg yolk protein concentrate[J]. International journal of food science & technology, 2010, 45: 1633-1640. DOI: 10.1111/j.1365-2621.2010.02311.x. [8]KO K, NAM K, JO C, et al.A simple and efficient method for preparing partially purified phosvitin from egg yolk using ethanol and salts[J]. Poultry science, 2011, 90: 1096-1104. DOI: 10.3382/ps.2010-01138. [9]SAMARAWEERA H, ZHANG W G, LEE E J, et al.Egg yolk phosvitin and functional phosphopeptides—Review[J]. Journal of food science i, 2011, 76: 143-150. DOI: 10.1111/j.1750-3841.2011.02291.x. [10]陈婵, 黄茜, 马美湖.鸡蛋中抗氧化活性蛋白质研究进展[J].中国家禽, 2015, 37(21):43-47 [11]CASTELLANI O, Guérin-Dubiard C, David-Briand E, et al.Influence of physicochemical conditions and technological treatments on the iron binding capacity of egg yolk phosvitin[J]. Food chemistry, 2004, 85: 569-577. DOI: 10.1016/j.foodchem.2003.08.002. [12]LI R, SHEN Y, ZHANG X, et al.Efficient purification of ginkgolic acids from Ginkgo biloba leaves by selective adsorption on Fe3O4 magnetic nanoparticles[J]. Journal of natural products, 2014, 77: 571-575. DOI: 10.1021/np400821r. [13]张松.Fe3O4磁性纳米粒子对黄酮类化合物的选择性吸附研究[D]. 长沙: 湖南师范大学图书馆, 2012: 1-8. [14]FAN J P, XU X K, XU R, et al.Preparation and characterization of molecular imprinted polymer functionalized with core/shell magnetic particles (Fe3O4@ SiO2@ MIP) for the simultaneous recognition and enrichment of four taxoids in Taxus× media[J]. Chemical Engineering Journal, 2015, 279: 567-577. DOI: 10.1016/j.cej.2015.05.045. [15]WU H T, HSU C C, TSAI C F, et al.Nanoprobe-based immobilized metal affinity chromatography for sensitive and complementary enrichment of multiply phosphorylated peptides[J]. Proteomics, 2011, 11: 2639-2653. DOI: 10.1002/pmic.201000768. [16]SUN J, LIU Y, SU Y, et al.Highly efficient enrichment of phosvitin phosphopeptides by novel magnetic carboxymethyl chitosan nanoparticles decorated with Fe (III) ions[J]. Journal of Chromatography B, 2013, 915: 33-38. DOI: 10.1016/j.jchromb.2012.12.013. [17]张婧.卵黄高磷蛋白磷酸肽的分离纯化及功能性质研究 [D]. 无锡: 江南大学, 2012: 9-10. [18]PIROUZ M J, BEYKI M H, SHEMIRANI F.Anhydride functionalised calcium ferrite nanoparticles A new selective magnetic material for enrichment of lead ions from water and food samples[J]. Food Chemistry, 2015, 170: 131-137. DOI: 10.1016/j.foodchem.2014.08.046. [19]王文娟, 张海鹏, 韩玉香, 等.磁性@@对水中磷酸盐的吸附研究[J].无机化学学报, 2014, 30(12):2726-2732 [20]张燚.Fe3O4磁性纳米粒子-氧化石墨烯复合材料的可控制备与应用[D]. 西安: 陕西师范大学, 2011: 1-6. [21]CHEN T T, YANG W J, GUO R J, et al.Enhancing catalytic performance of β-glucosidase via immobilization on metal ions chelated magnetic nanoparticles[J]. Enzyme and Microbial Technology, 2014, 63: 50-57. DOI: 10.1016/j.enzmictec.2014.05.008. [22]CHEN Z M, GENG Z R, Zhang Z Y, et al.Synthesis of Magnetic Fe3O4@C Nanoparticles Modified with -SO3H and -COOH Groups for Fast Removal of Pb2+, Hg2+, and Cd2+ Ions[J]. European Journal of Inorganic Chemistry, 20: 3172-3177. DOI: 10.1002/ejic.201301500. [23]孙海平.磁性纳米粒子的表面修饰及功能化 [D].扬州: 扬州大学, 2008: 32-46. [24]ZHANG J, SUN J, LIU Y, et al.Separation and purification of phosvitin phosphopeptides using immobilized metal affinity nanoparticles[J]. Journal of Chromatography B, 2012, 893: 121-126. DOI: 10.1016/j.jchromb.2012.02.046. [25]KAMRAN S, ASADI M, ABSALAN G.. Adsorption of acidic, basic, and neutral proteins from aqueous samples using Fe3O4 magnetic nanoparticles modified with an ionic liquid[J]. Microchimica Acta, 2013, 180: 41-48. DOI: 10.1007/s00604-012-0901-7. [26]ZHOU L X, PAN S D, CHEN X H, et al.Kinetics and thermodynamics studies of pentachlorophenol adsorption on covalently functionalized Fe3O4@SiO2-MWCNTs core–shell magnetic microspheres[J]. Chemical Engineering Journal, 2014, 257: 10-19. DOI: 10.1016/j.cej.2014.07.060.