• 成分分析 •

### 近红外漫反射光谱法快速检测谷子蛋白质和淀粉含量

1. （1.山西省农业科学院农作物品种资源研究所，山西?太原 030031；2.农业部黄土高原作物基因与种质创制重点实验室，山西?太原 030031；3.杂粮种质资源发掘与遗传改良山西省重点实验室，山西?太原 030031）
• 出版日期:2017-08-25 发布日期:2017-08-18
• 基金资助:
国家谷子高粱产业技术体系谷子糜子生理岗位项目（CARS-06-13.5-A16）；国家农作物种质资源平台山西作物子平台项目（NICGR2016-02）；山西省农作物种质资源鉴定评价研究项目（2017ZZCX-17）；山西省省级财政支农专项（2016zyzx41）

### Application of Near Infrared Diffuse Reflectance Spectroscopy in Rapid Detection of Crude Protein and Starch in Foxtail Millet

TIAN Xiang,, LIU Sichen,, WANG Haigang,, QIN Huibin,, QIAO Zhijun,

1. (1. Institute of Crop Germplasm Resources, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China; 2. Key Laboratory of Crop Gene Resources & Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030031, China; 3. Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan 030031, China)
• Online:2017-08-25 Published:2017-08-18

Abstract: This study aimed to establish a near infrared (NIR) diffuse reflectance spectrometry method for measuring the contents of crude protein and starch in foxtail millet for providing a fast, simple and non-destructive analytical method for the identification and screening of foxtail millet germplasm resources. For this purpose, a total of 191 samples of a foxtail millet core collection in Shanxi province were measured using NIR diffuse reflectance spectroscopy. The results showed that with the spectral preprocessing method of first derivative + vector normalization, calibration models were established to predict the contents of crude protein and starch in foxtail millet. The corresponding coefficients of determination for calibration (R2cal) were 0.977 0 and 0.907 3, and the root mean square errors of cross-validation (RMSECV) were 0.203% and 0.466%, the coefficients of determination for external validation (R2val) were 0.989 6 and 0.977 2, and the root mean square errors of prediction (RMSEP) were 0.225% and 0.368%, respectively. For the detection of crude protein and starch foxtail millet, no significant differences were seen between chemical analysis and NIR measurement, but the results obtained with NIR measurement were more accurate and reliable. NIR diffuse reflectance spectrometry technology can be useful for the detection of crude protein and starch in foxtail millet.