食品科学 ›› 2017, Vol. 38 ›› Issue (15): 183-188.doi: 10.7506/spkx1002-6630-201715030

• 基础研究 • 上一篇    下一篇

亲和毛细管电泳法和荧光猝灭法分析α-乳白蛋白与离子液体相互作用

贾娜尔·吐尔逊,王晓娅,谭瑞康,李德强   

  1. (1.新疆农业大学化学工程学院,新疆?乌鲁木齐 830052;2.新疆蓝山屯河能源有限公司质管部,新疆?昌吉 831800)
  • 出版日期:2017-08-15 发布日期:2017-09-06
  • 基金资助:
    新疆农业大学校前期资助课题(XJAU201204)

Interactions of α-Lactalbumin with Ionic Liquids Analyzed by Affinity Capillary Electrophoresis and Fluorescence Quenching Method

TUERXUN Jianaer, WANG Xiaoya, TAN Ruikang, LI Deqiang   

  1. (1. College of Chemical Engineering, Xinjiang Agricultural University, ürümqi 830052, China; 2. Quality Control Department, Xinjiang Blue Ridge Tunhe Chemical Industry Joint Stock Co. Ltd., Changji 831800, China)
  • Online:2017-08-15 Published:2017-09-06

摘要: 以溴化1-乙基-3-甲基咪唑(1-ethyl-3-methylimidazolium bromide,[C2mim]Br)为原料,采用中和法合成氨基酸离子液体1-乙基-3-甲基咪唑-2-氨基-1,3-戊二酸盐(1-ethyl-3-methylimidazolium 1-aminopropane- 1,3-dicarboxylic acid salt,[C2mim][Glu])。分别采用亲和毛细管电泳法和荧光猝灭法考察α-乳白蛋白与[C2mim][Glu]、α-乳白蛋白与[C2mim]Br的相互作用。结果得到二者结合常数均在106?L/mol量级,且α-乳白蛋白-[C2mim][Glu]的结合常数大于α-乳白蛋白-[C2mim]Br,结合比约为1∶1。实验结果为进一步研究α-乳白蛋白与离子液体相互作用机理、开发高效环境友好的α-乳白蛋白萃取体系具有一定的参考意义。

关键词: α-乳白蛋白, 氨基酸离子液体, 亲和毛细管电泳, 结合常数, 荧光猝灭法

Abstract: An amino acid ionic liquid (AAIL), 1-ethyl-3-methylimidazolium 1-aminopropane-1,3-dicarboxylic acid salt ([C2mim][Glu]), was synthesized by the neutralization method from 1-ethyl-3-methylimidazolium bromide ([C2mim]Br). The binding behavior of α-lactalbumin (α-LAB) with [C2mim][Glu] and [C2mim]Br was evaluated by affinity capillary electrophoresis and fluorescence quenching method. The binding constants of both α-LAB-[C2mim][Glu] and α-LAB-[C2mim]Br were in the order of magnitude of 106 L/mol, with the former being higher than the latter, and the binding ratios were approximately 1:1. This research may provide some references for further studies aimed to understand the mechanism behind α-LAB-ionic liquid interaction and develop an efficient and environment-friendly extraction system for α-LAB.

Key words: α-lactalbumin (α-LAB), amino acid ionic liquids (AAIL), affinity capillary electrophoresis (ACE), binding constant, fluorescence quenching method

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