食品科学 ›› 2017, Vol. 38 ›› Issue (17): 233-237.doi: 10.7506/spkx1002-6630-201717038

• 营养卫生 • 上一篇    下一篇

酵母源金属硫蛋白排汞及对汞致肝脏及机体氧化损伤的修复作用

王欣卉1,王 颖1,2,*,徐炳政3,佐兆杭1,刘淑婷1,宫 雪1,张东杰1,*   

  1. 1.黑龙江八一农垦大学食品学院,黑龙江 大庆 163319;2.国家杂粮工程技术研究中心,黑龙江 大庆 163319;3.青岛琅琊台集团股份有限公司,山东 青岛 266400
  • 出版日期:2017-09-15 发布日期:2017-09-12
  • 基金资助:
    黑龙江省博士后落地资助项目(LBH-Q15116);黑龙江省新世纪优秀人才培养计划项目(1254-NCET-015);黑龙江八一农垦大学青年创新人才项目(CXRC2016-15);新世纪优秀人才项目(1254-NCET-015);2015年度国家星火计划项目(2015GA670008);黑龙江八一农垦大学研究生创新科研项目(YJSCX2017-Y56;YJSCX2017-Y57)

Effect of Yeast Metallothioneins on Mercury Elimination and Repairing of Mercury-Induced Acute Body and Liver Injury

WANG Xinhui1, WANG Ying1,2,*, XU Bingzheng3, ZUO Zhaohang1, LIU Shuting1, GONG Xue1, ZHANG Dongjie1,*   

  1. 1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. National Coarse CerealsEngineering Research Center, Daqing 163319, China; 3. Qingdao Langyatai Co. Ltd., Qingdao 266400, China
  • Online:2017-09-15 Published:2017-09-12

摘要: 目的:探讨两种酵母源金属硫蛋白(metallothioneins,MT)-1、MT-2对氯化汞急性汞中毒小鼠排汞及对汞致肝脏及机体氧化损伤的修复作用。方法:氯化汞急性汞中毒小鼠模型通过颈部皮下注射氯化汞生理盐水溶液构建,急性汞中毒小鼠经连续灌胃二巯基丙磺酸钠(dimercaptopropansulfonate sodium,DMPS)及不同剂量(0.16、0.40、0.80 mg/(kg·d))酵母源MT 14 d后,测定小鼠体质量、肝脏脏器系数、血液及肝脏Hg2+的含量、血清谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力、超氧化物歧化酶(superoxide dismutase,SOD)活力、机体总抗氧化能力(total antioxidative capacity,T-AOC)及丙二醛(malondialdehyde,MDA)含量和两项肝功能指示指标:谷丙转氨酶(alanine aminotransferase,ALT)及谷草转氨酶(aspartate transaminase,AST)活力。结果:相较于正常组小鼠,模型组小鼠体质量下降、肝脏脏器系数上升,差异具有统计学意义(P<0.05);血液及肝脏Hg2+含量显著上升(P<0.05);血清GSH-Px、SOD活力及T-AOC显著降低(P<0.05),MDA含量显著升高(P<0.05);两项肝功能指标ALT及AST活力显著升高(P<0.05)。经灌胃给药处理后,与模型组小鼠相比,酵母源MT中、高剂量处理组及二巯基丙磺酸钠阳性对照组小鼠体质量均显著上升(P<0.05),并与正常组小鼠无显著性差异(P>0.05);各给药处理组小鼠脏器系数均较模型组小鼠显著下降(P<0.05)且呈一定的剂量-效应关系。各灌胃给药处理组小鼠血液及肝脏Hg2+含量均不同程度下降;血清GSH-Px、SOD活力及T-AOC均不同程度回升,MDA含量及两项肝功能指标ALT及AST活力均不同程度降低。在0.16~0.80 mg/kg剂量范围内,高剂量酵母源MT给药效果最佳,与其他灌胃给药处理组差异显著(P<0.05)。结论:两种酵母源MT对急性汞中毒小鼠具有良好的结合机体内游离Hg2+及修复肝脏损伤及机体氧化损伤作用,且作用效果与给药剂量呈正相关。

关键词: 酵母源金属硫蛋白, 排汞, 修复, 肝脏, 氧化损伤

Abstract: Objective: To investigate the effect of two yeast metallothioneins (MT-1 and MT-2) on mercury elimination and the repairing of liver damage and oxidative damage in mice with acute mercury chloride poisoning. Methods: A mouse model of acute mercury poisoning was established by subcutaneous injection of mercuric chloride in normal saline in the neck. The acute mercury poisoning mice were lavaged with each MT at different doses or DMPS for 14 continuous days. At the end of the treatment period, the body weight, liver coefficient, mercury levels in blood and liver, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, malondialdehyde (MDA) content and total antioxidant capacity (T-AOC) in serum, and liver alanine aminotransferase (ALT) and aspartate transaminase (AST) activities were measured. Results: Compared with the normal group, body weight in the model group was significantly decreased (P < 0.05), and liver coefficient was increased (P < 0.05); serum mercury levels were increased significantly (P < 0.05); SOD and serum GSH-Px activities and T-AOC were decreased significantly (P < 0.05); serum MDA level and liver ALT and AST activities were increased significantly (P < 0.05). Yeast MT at middle and high doses and the positive control dimercaptopropansulfonate sodium resulted in significantly higher body weight compared with the model group (P < 0.05), and exhibited no significant difference from the normal group (P > 0.05). The treatments resulted in significantly dose-dependently reduced liver coefficient in comparison with the model group (P < 0.05). For all treatment groups, Hg2+ levels in serum and liver, serum MDA level, and liver ALT and AST activities were decreased to different extents, and serum GSH-Px and SOD activities and T-AOC were increased to different extents. High-dose yeast metallothioneins had the best effect in the dose range of 0.16–0.80 mg/kg, and the effect was significantly different from that of other groups (P < 0.05). Conclusion: Both yeast MT could significantly dose-dependently bind free Hg2+ in mice with acute mercury chloride poisoning and repair liver damage and oxidative damage.

Key words: yeast metallothioneins, mercury elimination, repairing, liver, oxidative damage

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