食品科学 ›› 2017, Vol. 38 ›› Issue (17): 238-243.doi: 10.7506/spkx1002-6630-201717039

• 营养卫生 • 上一篇    下一篇

植物乳杆菌YS-1对活性炭诱导小鼠便秘的预防作用

易若琨,骞 宇,王 强,母健菲,赵 欣*   

  1. 重庆第二师范学院生物与化学工程系,重庆市功能性食品协同创新中心,重庆市功能性食品工程技术研究中心,功能性食品研发重庆市工程实验室,重庆 400067
  • 出版日期:2017-09-15 发布日期:2017-09-12
  • 基金资助:
    重庆高校创新团队建设计划资助项目(CXTDX201601040);重庆市基础科学与前沿技术研究项目(cstc2016jcyjA0339);重庆第二师范学院科研项目(KY2015TBZC)

Preventive Effect of Lactobacillus plantarum YS-1 on Activated Carbon-Induced Constipation in Mice

YI Ruokun, QIAN Yu, WANG Qiang, MU Jianfei, ZHAO Xin*   

  1. Chongqing Collaborative Innovation Center for Functional Food, Chongqing Engineering Research Center of Functional Food,Chongqing Engineering Laboratory for Research and Development of Functional Food,Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
  • Online:2017-09-15 Published:2017-09-12

摘要: 检测植物乳杆菌YS-1(Lactobacillus plantarum YS-1,LP-YS1)对活性炭诱导便秘昆明小鼠的影响。结果表明LP-YS1的抗胃酸和胆盐能力强于保加利亚乳杆菌。LP-YS1能抑制便秘造成的小鼠体质量、粪便质量、颗粒数和含水量的下降。同时LP-YS1可以提高活性炭在小肠中的推进率和缩短排出首粒黑便的时间。LP-YS1还能使便秘小鼠的胃动素(motilin,MTL)、内皮素(endothelin,ET)、乙酰胆碱酯酶(acetylcholinesterase,AChE)、P物质(substance P,SP)、血管活性肠肽(vasoactive intestinal peptide,VIP)水平提高和生长抑素(somatostatin,SS)水平下降。逆转录聚合酶链式反应实验进一步显示LP-YS1可以上调便秘小鼠小肠组织c-Kit(干细胞因子受体)、干细胞因子(stem cell factor,SCF)、胶质细胞源性神经营养因子(glial cellline-derived neurotrophic factor,GDNF)基因的mRNA表达和下调瞬时感受器电位香草酸受体1(transient receptor potential vanilloid 1,TRPV1)、一氧化氮合酶(nitric oxide synthase,NOS)基因的表达。高浓度的LP-YS1显示出更好的作用,且显著优于保加利亚乳杆菌。这些实验结果证明LP-YS1可有效缓解便秘。

关键词: 植物乳杆菌, 活性炭, 便秘, 胃动素, 内皮素, 乙酰胆碱酯酶

Abstract: The aim of this study was to determine the effect of Lactobacillus plantarum YS-1 (LP-YS1) on activated carboninduced constipation in Kunming mice. The experimental results showed that anti-gastric acid and bile salt activities of LP-YS1 were stronger than that of Lactobacillus bulgaricus. LP-YS1 could inhibit the decrease in body weight, fecal weight, fecal pellet number and fecal moisture content caused by constipation in mice. Meanwhile, LP-YS1 could raise gastrointestinal (GI) transit rate and reduce the time to the first black stool defecation. LP-YS1 also could increase serum motilin (MTL), endothelin (ET), acetylcholinesterase (AChE), substance P (SP), and vasoactive intestinal peptide (VIP) levels and decrease somatostatin (SS) level in constipated mice. reverse transcription polymerase chain reaction experiments revealed that LP-YS1 could raise c-Kit, stem cell factor (SCF) and glial cellline-derived neurotrophic factor (GDNF) mRNA expression and reduce transient receptor potential vanilloid 1 (TRPV1) and nitric oxide synthase (NOS) mRNA expression in the small intestine of constipated mice. High concentration of LP-YS1 had a better effect. Based on these results, LP-YS1 could effectively inhibit constipation.

Key words: Lactobacillus plantarum, activated carbon, constipation, motilin, endothelin, acetylcholinesterase

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