环糊精功能化修饰纳米银负载姜黄素的制备及其抗肿瘤活性

doi:10.7506/spkx1002-6630-201721006

食品科学 ›› 2017, Vol. 38 ›› Issue (21): 38-42.doi: 10.7506/spkx1002-6630-201721006

环糊精功能化修饰纳米银负载姜黄素的制备及其抗肿瘤活性

陈建平，余苗，秦小明，谌素华，钟赛意

（1.广东海洋大学食品科技学院，广东?湛江 524088；2.广东省天然产物绿色加工与产品安全重点实验室，广东?广州 510640； 3.江西省粮油质量监督检验中心，江西?南昌 330046）

出版日期:2017-11-15 发布日期:2017-11-01
基金资助:
国家自然科学基金青年科学基金项目（21602034）；广东省自然科学基金博士启动项目（2016A30310332）；广东省公益研究与能力建设项目（2015A020209166）；广东省天然产物绿色加工与产品安全重点实验室开放基金项目（201617）

Preparation and Anticancer Activity of Cyclodextrin-Functionalized Curcumin-Loaded Silver Nanoparticles

CHEN Jianping, YU Miao, QIN Xiaoming, CHEN Suhua, ZHONG Saiyi

(1. College of Food and Technology, Guangdong Ocean University, Zhanjiang 524088, China; 2. Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China; 3. Grain and Edible Oil Products Quality’s Supervision and Inspection Center in Jiangxi Province, Nanchang 330046, China)

Online:2017-11-15 Published:2017-11-01

摘要/Abstract

摘要： 采用纳米银材料制备负载姜黄素（curcumin，Cur）的β-环糊精（β-cyclodextrin，β-CD）功能化纳米银——Ag@β-CD@Cur，并进一步对其抗肿瘤活性进行测定。运用透射电子显微镜和纳米粒度仪鉴定Ag@β-CD@Cur的表面形貌、粒径大小和稳定性。运用倒置荧光显微镜和3-（4,5-二甲基噻唑-2）-2,5-二苯基四氮唑溴盐法检测Ag@β-CD@Cur对人肝癌细胞HepG2形态和活性的影响，并进一步采用流式细胞术检测细胞凋亡比例。实验结果表明，所制备的Ag@β-CD@Cur是纯度很高的单质银，且大小均一、表面光滑，粒径约为2?nm。同时，Ag@β-CD@Cur具有较好的稳定性，能在水中稳定存在30?d。经Ag@β-CD@Cur处理HepG2细胞24?h后，HepG2细胞的形态出现明显的变圆、细胞核固缩等细胞凋亡特征，同时，细胞存活率从对照组的100%降低到24%，进一步的流式细胞术分析结果表明，细胞凋亡比例从对照组的2.5%增加到51.0%。实验结果表明，纳米化后的Cur具有较强的抗肿瘤活性效果，这为Cur在纳米制剂的应用提供了一定的技术手段和理论数据参考。

关键词: 姜黄素, β-环糊精, 银纳米粒子, 制备, 抗肿瘤活性

Abstract: In this study, cyclodextrin-functionalized curcumin-loaded silver nanoparticles (Ag@β-CD@Cur) were prepared with silver nanomaterials and its anticancer activity was also studied. The surface morphology, particle size and stability of Ag@β-CD@Cur were detected by transmission electronic microscopy and Zetasizer Nano. Moreover, the effect of Ag@β-CD@Cur on morphological and viability changes of HepG2 cells were detected by phase-contrast microscopy and (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Moreover, the population of apoptotic cells was detected by flow cytometric analysis. The experimental results showed that Ag@β-CD@Cur was obtained as highly pure, uniformly sized silver nanoparticles with a smooth surface. The average size of Ag@β-CD@Cur was 2 nm. Moreover, Ag@β-CD@Cur had good stability in water for 30 days. After treatment of HepG2 cells with Ag@β-CD@Cur for 24 h, apoptosis characteristics appeared such as cell swelling and cell nuclei pyknosis. At the same time, the cell viability reduced to 24% of the control group. Further flow cytometric analysis showed that the population of apoptotic cells increased from 2.5% (control) to 51.0%. The results demonstrated that after nanoparticlization, curcumin has stronger anticancer activity, which will provide a technical means and theoretical data for curcumin nano-formulation.

Key words: curcumin, β-cyclodextrin, silver nanoparticles, preparation, anticancer activity

中图分类号:

TS201.2

陈建平，余苗，秦小明，谌素华，钟赛意. 环糊精功能化修饰纳米银负载姜黄素的制备及其抗肿瘤活性[J]. 食品科学, 2017, 38(21): 38-42.

CHEN Jianping, YU Miao, QIN Xiaoming, CHEN Suhua, ZHONG Saiyi. Preparation and Anticancer Activity of Cyclodextrin-Functionalized Curcumin-Loaded Silver Nanoparticles[J]. FOOD SCIENCE, 2017, 38(21): 38-42.

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环糊精功能化修饰纳米银负载姜黄素的制备及其抗肿瘤活性

Preparation and Anticancer Activity of Cyclodextrin-Functionalized Curcumin-Loaded Silver Nanoparticles

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