食品科学 ›› 2017, Vol. 38 ›› Issue (21): 170-174.doi: 10.7506/spkx1002-6630-201721027

• 营养卫生 • 上一篇    下一篇

市售鸡肉及内脏中磺胺耐药菌污染特征

李姝,邵毅,周昌艳,王华,,邢增涛,5,赵志辉   

  1. (1.上海海洋大学食品学院,上海 201306;2.上海市农业科学院农产品质量标准与检测技术研究所,上海 201403;3.美国俄亥俄州立大学食品科学与技术系,美国?俄亥俄?哥伦布 43210;4.复旦大学生命科学院微生物系,上海 200433;5.上海市农业委员会,上海 200003)
  • 出版日期:2017-11-15 发布日期:2017-11-01
  • 基金资助:
    国家自然科学基金青年科学基金项目(31401599);上海市科技兴农重点攻关项目(应用基础类)(沪农科攻字(2014)第7-3-6号);上海市青年科技英才扬帆计划项目(14YF1413000);美国外交部国际教育协会美英联合全球创新倡议(GII)项目(GRT00033913)

Prevalence and Characteristics of Sulfamethoxazole Resistant Bacteria in Retail Chicken Meat and Giblets

LI Shu, SHAO Yi, ZHOU Changyan, WANG Hua, , XING Zengtao,, ZHAO Zhihui   

  1. (1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 2. Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; 3. Department of Food Science and Technology, The Ohio State University, Columbus 43210, USA; 4. Department of Microbiology, School of Life Sciences, Fudan University, Shanghai 200433, China; 5. Shanghai Agriculture Committee, Shanghai 200003, China)
  • Online:2017-11-15 Published:2017-11-01

摘要: 为评价抗生素耐药基因通过市售鸡肉产品进行迁移的风险,从上海5?个大型超市和综合市场采集的9?个鸡肉样品和4?个内脏样品中分离对磺胺甲噁唑/甲氧苄啶不敏感的细菌(Sul/Trir),研究其耐药特征,并用Southern杂交对耐药基因进行定位。结果表明,所有样品均分离到了Sul/Trir菌,菌落数为2.0×102~1.0×107?CFU/g,占总可培养菌的1.42%~82.00%。297?株Sul/Trir菌的多重耐药现象普遍,耐药基因sul1、sul2、tetA、tetB、tetC、tetE、tetG、tetL、tetM和intI的检出率分别为11.33%、32.81%、6.64%、12.89%、2.73%、6.25%、9.38%、18.75%、39.84%和28.52%。耐药基因的寄主菌包括Escherichia sp.、Pedobacter sp.、Staphylococcus sp.、Enterococcus sp.、Sphingobacterium sp.和Acinetobacter sp.等,大多数的磺胺最小抑菌浓度大于512 μg/mL,其中一株葡萄球菌的质粒上有sul2基因。综上,耐药菌和耐药基因可能通过市售鸡肉及内脏产品进行迁移。

关键词: 磺胺耐药菌, 耐药基因, 鸡肉, 内脏

Abstract: In order to investigate the potential risk of retail chicken products in transmitting antibiotic resistance (AR) encoding genes, sulfamethoxazole/trimethoprim resistant (Sul/Trir) bacteria were isolated from 13 chicken products, including nine chicken meat samples and four giblet samples, purchased from five supermarkets and local markets. The Sul/Trir resistance genes were mapped by Southern blot analysis. Sul/Trir bacteria were detected in all chicken samples. The prevalence of Sul/Trir bacteria ranged from 2.0 × 102 CFU/g to 1.0 × 107 CFU/g, accounting for 1.42%–82.00% of the total cultivable bacteria. Multi-drug resistance was observed for all 297 Sul/Trir isolates, and the positive rates of AR genes including sul1, sul2, tetA, tetB, tetC, tetE, tetG, tetL, tetM and intI were 11.33%, 32.81%, 6.64%, 12.89%, 2.73%, 6.25%, 9.38%, 18.75%, 39.84% and 28.52% respectively. Escherichia sp., Pedobacter sp., Staphylococcus sp., Enterococcus sp., Sphingobacterium sp. and Acinetobacter sp. were identified as hosts for AR genes. The minimum inhibitory concentration (MIC) of Sul for most isolates was higher than 512 μg/mL. Furthermore, a plasmid harboring sul2 gene was found in one Sul/Trir isolate by Southern blot hybridization. In conclusion, retail chicken products can be a potential avenue transmitting antibiotic resistant bacteria and AR genes.

Key words: sulfamethoxazole/trimethoprim resistant bacteria, antibiotic resistance genes, chicken meat, giblets

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