食品科学 ›› 2017, Vol. 38 ›› Issue (21): 230-240.doi: 10.7506/spkx1002-6630-201721037

• 营养卫生 • 上一篇    下一篇

干酪乳杆菌Qian增强京尼平苷对盐酸/乙醇诱导小鼠胃损伤的预防效果

骞宇,索化夷,易若琨,李贵节,赵欣   

  1. (1.重庆第二师范学院生物与化学工程学院,重庆市功能性食品协同创新中心,重庆市功能性食品工程技术研究中心,功能性食品研发重庆市工程实验室,重庆 400067;2. 西南大学食品科学学院,重庆 400715)
  • 出版日期:2017-11-15 发布日期:2017-11-01
  • 基金资助:
    重庆高校创新团队建设计划项目(CXTDX201601040); 重庆市工程技术研究中心建设项目(cstc2015yfpt_gcjsyjzx0027); 重庆市基础与前沿研究计划一般项目(cstc2016jcyjA0339);重庆第二师范学院校级科研项目(KY2015TBZC)

Lactobacillus casei Qian Enhances the Preventive Effect of Geniposide on HCl/Ethanol Induced Gastric Injury in Mice

QIAN Yu, SUO Huayi, YI Ruokun, LI Guijie, ZHAO Xin   

  1. (1. Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Collaborative Innovation Center for Functional Food, College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China; 2. College of Food Science, Southwest University, Chongqing 400715, China)
  • Online:2017-11-15 Published:2017-11-01

摘要: 为探讨最近新发现的干酪乳杆菌Qian(Lactobacillus casei Qian,LC-Qian)和京尼平苷组合对胃损伤预防效果的增强作用,比较分析LC-Qian(0.5×108?CFU/kg,以体质量计,下同)、京尼平苷(50?mg/kg)以及LC-Qian(0.5×108?CFU/kg)+京尼平苷(50?mg/kg)对盐酸/乙醇诱导胃损伤的预防效果。LC-Qian+京尼平苷(组合组)能减少胃损伤面积和胃液量,同时提高胃液pH值,能使这些指标接近正常组并且与模型组显著不同。相对于模型组,组合组能降低细胞因子白细胞介素-6(interleukin-6,IL-6)、IL-12、肿瘤坏死因子α和γ-干扰素的水平,同时降低血清胃动素、P物质、内皮素-1的水平和提高生长激素抑制素、血管活性肠肽的水平。相比模型组,在心脏、肝脏、肾脏和胃组织中组合组表现出更高的NO含量,但是这些含量低于正常组。通过逆转录聚合酶链式反应分析观察到:相对于对照组小鼠,组合组能够上调神经元型一氧化氮合酶、内皮型一氧化氮合酶、核因子κB抑制蛋白α的mRNA表达和下调诱导型一氧化氮合成酶、核因子κB表达,同时以上这些表达强度与正常组较为接近。通过这些结果可以看到LC-Qian和京尼平苷联合使用具有最好的胃损伤预防效果,LC-Qian能够提高京尼平苷的胃损伤预防效果,该组合可作为一种新的参考方法用于预防胃损伤。

关键词: 干酪乳杆菌Qian, 乙醇, mRNA表达, 小鼠,

Abstract: The aim of this study was to investigate the synergistic effect of a recently discovered strain Lactobacillus casei Qian (LC-Qian) and geniposide on the prevention of ethanol-induced gastric injury in mice. LC-Qian (0.5 × 108 CFU/kg mb) was mixed with geniposide (50 mg/kg mb), and LC-Qian (0.5 × 108 CFU/kg mb) and geniposide (50 mg/kg mb) were also used separately to protect against HCl/ethanol induced gastric injury and to compare their preventive effects. LC-Qian combined with geniposide (the combination group) diminished the gastric lesion area and gastric secretion volume and raised the pH of the gastric juice to a level close of that of the control group and significantly different from that in the model group. The combination treatment was also able to decrease the serum levels of cytokines such as interleukin-6 (IL-6), IL-12, tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), motilin (MOT), substance P (SP), and endothelin-1 (ET-1) but increase the levels of somatostatin (SS) and vasoactive intestinal peptide (VIP) as compared to the model group. The combination group showed higher NO contents in serum, heart, liver, kidney and stomach in comparison to the model group, but their levels were lower than those in the normal group. As indicated by reverse transcription polymerase chain reaction (RT-PCR) assay, the combination treatment could increase the mRNA expression levels of neuronal nitric oxide synthase (nNOS), endothelial NOS (eNOS) and inhibitor kappa B-α (IκB-α), and decrease the mRNA expression levels of inducible nitric oxide synthase NOS (iNOS) and nuclear factor kappa B (NF-κB) as compared to the model group. These expression levels in the combination group were similar to those of the normal group. From these results, it can be concluded that the combination treatment has the best preventive effects against gastric injury and LC-Qian could enhance the preventive effect of geniposide against gastric injury. Therefore, this combination treatment could be used as a new way method for the prevention of gastric injury.

Key words: Lactobacillus casei Qian, ethanol, mRNA expression, mouse, stomach

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