食品科学 ›› 2017, Vol. 38 ›› Issue (24): 229-234.doi: 10.7506/spkx1002-6630-201724037

• 安全检测 • 上一篇    下一篇

PCR技术鉴别多趾北京油鸡肉

陈静茹,夏海剑,张泽宾,曲鲁江,刘毅,李兴民   

  1. (1.中国农业大学食品科学与营养工程学院,北京 100083;2.中国农业大学动物科技学院,北京 100094)
  • 出版日期:2017-12-25 发布日期:2017-12-07
  • 基金资助:
    北京市家禽产业创新团队项目(BAIC04-2017)

Identification of Multi-Toed Beijing-You Chicken by PCR

CHEN Jingru, XIA Haijian, ZHANG Zebin, QU Lujiang, LIU Yi, LI Xingmin   

  1. (1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;2. College of Animal Science & Technology, China Agricultural University, Beijing 100094, China)
  • Online:2017-12-25 Published:2017-12-07

摘要: 北京油鸡是我国珍贵的地方品种,在自然群体中,部分个体表现出多趾性状,该性状是由于其LMBR1基因的246bp处发生突变,突变位点的碱基由C突变为A时表现出多趾形状。本实验根据多趾北京油鸡肉基因型与华都艾拔益加肉鸡基因型的不同,通过聚合酶链式反应(polymerase chain reaction,PCR)测序法和限制性酶切片段多态性(restriction fragment length polymorphism,RFLP)技术,确定突变位点的碱基为A或C,从而准确鉴定食品中多趾北京油鸡肉与华都艾拔益加肉鸡的区别。

关键词: 北京油鸡, 艾拔益加肉鸡, 多趾, PCR测序, 限制性酶切片段多态性(RFLP)

Abstract: Beijing-you chicken is one of the precious local breeds in China. In the natural population, there are some multi-toed individuals because of the mutation of C-G base pair to an A-U pair at the 246 bp site in the LMBR1 gene. In this study, the genotype of multi-toed Beijing-you chicken was found to be different from that of Huadu Arbor Acres (AA) broiler, and the mutated base pair (A-U or C-G) was determined by PCR sequencing and restriction fragment polymorphism (RFLP) so that the breeds could be accurately discriminated from each other.

Key words: Beijing-you chicken, Huadu Arbor Acres broiler, polydactly, PCR sequencing, restriction fragment polymorphism

中图分类号: