SNP标记用于猪肉产品DNA溯源

doi:10.7506/spkx1002-6630-201724045

食品科学 ›› 2017, Vol. 38 ›› Issue (24): 278-282.doi: 10.7506/spkx1002-6630-201724045

SNP标记用于猪肉产品DNA溯源

吴潇，吕贝贝，王金斌，蒋玮，李鹏，武国干，唐雪明

（上海市农业科学院生物技术研究所，上海 201106）

出版日期:2017-12-25 发布日期:2017-12-07
基金资助:
闵行区产学研合作计划项目（2016MH256）

Development and Application of Pork Traceability System Based on SNP Markers

WU Xiao, Lü Beibei, WANG Jinbin, JIANG Wei, LI Peng, WU Guogan, TANG Xueming

(Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China)

Online:2017-12-25 Published:2017-12-07

摘要/Abstract

摘要： 采用基于个体基因组DNA序列的差异而进行个体识别的DNA溯源技术，建立DNA溯源系统对肉产品的质量安全进行控制。在实验群体中（10?个品种，233?个个体）检测了33?个新单核苷酸多态性（single nucleotide polymorphism，SNP）标记的遗传多样性，通过杂合度计算筛选出6?个SNP标记可用于猪肉产品DNA溯源。进一步在屠宰场采样进行溯源模拟实验，结果表明筛选的18?个SNP标记（6?个新SNP标记结合已有的12?个SNP标记）能有效区分100?头猪个体，随机抽取的10?个个体的组织样品都能通过基因型比对找到对应的个体。本研究可为早日建立猪肉产品的DNA溯源系统提供一定的技术参考。

关键词: 猪肉产品溯源, DNA溯源技术, SNP标记, 个体识别

Abstract: DNA traceability technology is based on DNA sequence differences between individuals for individual identification and the tracing of meat back to an individual animal. Establishing a DNA-based traceability system is important for the safety and quality of meat products. The genetic diversity of 33 new single nucleotide polymorphism (SNP) markers was detected in a test group (233 individuals, 10 varieties) and 6 SNP markers, which are useful for pork product traceability, were obtained by heterozygosity calculation. A total of 18 SNP markers (6 new SNP markers in combination with 12 SNP markers) could effectively distinguish between 100 individuals collected from a local slaughter house in traceability simulation experiments. The corresponding individuals for tissue samples from 10 random individuals were found by genotype. This study has shown that SNP markers could be used for individual identification and pork traceability.

Key words: pork traceability, DNA traceability technology, SNP markers, individual identification

中图分类号:

Q819

吴潇，吕贝贝，王金斌，蒋玮，李鹏，武国干，唐雪明. SNP标记用于猪肉产品DNA溯源[J]. 食品科学, 2017, 38(24): 278-282.

WU Xiao, Lü Beibei, WANG Jinbin, JIANG Wei, LI Peng, WU Guogan, TANG Xueming. Development and Application of Pork Traceability System Based on SNP Markers[J]. FOOD SCIENCE, 2017, 38(24): 278-282.

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SNP标记用于猪肉产品DNA溯源

Development and Application of Pork Traceability System Based on SNP Markers

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