脉冲强光对枯草芽孢杆菌NG-2灭活机理

doi:10.7506/spkx1002-6630-201723012

食品科学 ›› 2017, Vol. 38 ›› Issue (23): 70-74.doi: 10.7506/spkx1002-6630-201723012

脉冲强光对枯草芽孢杆菌NG-2灭活机理

管峰，徐雯钗，袁勇军*，张瑞雪，赵思敏，陈秋平

浙江万里学院生物与环境学院，浙江宁波 315100

出版日期:2017-12-15 发布日期:2017-12-07
基金资助:
浙江省重中之重学科“生物工程”学生创新项目（CX2016012）；宁波市高校院所研发投入补助项目（2011B22012）；宁波市江北区农业项目（2012B04）

Inactivation and Mechanism of Bacillus subtilis NG-2 and Its Spores by Pulsed Light

GUAN Feng, XU Wenchai, YUAN Yongjun*, ZHANG Ruixue, ZHAO Simin, CHEN Qiuping

College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China

Online:2017-12-15 Published:2017-12-07

摘要/Abstract

摘要： 采用脉冲强光对枯草芽孢杆菌NG-2及芽孢进行灭活并研究其机理。在脉冲强光辐照枯草芽孢杆菌NG-2菌体及其芽孢后，利用扫描电子显微镜和透射电子显微镜观察形态特征变化，利用紫外分光光度法测定细胞内物质含量，利用荧光光谱法检测细胞内Ca2＋浓度和非特异性酯酶活力。结果表明，辐照距离为5 cm，闪照频率为5 次/s，辐照15 s时，脉冲强光对NG-2菌体及其芽孢灭活率超过50%；辐照40 s时，脉冲强光对NG-2菌体及其芽孢灭活率超过99.98%。辐照枯草芽孢杆菌及芽孢后菌体结构损伤，芽孢壁破裂，细胞内核酸、蛋白质、Ca2＋和2,6-吡啶二羧酸含量显著减少，非特异性酯酶活力降低，研究结果为脉冲强光对枯草芽孢杆菌及芽孢灭活机理提供理论依据。

关键词: 脉冲强光, 枯草芽孢杆菌, 芽孢, 灭活机理

Abstract: Pulsed light was used for the inactivation of Bacillus subtilis NG-2 and its spores. Morphological characteristics of the irradiated cells and spores were explored by scanning electron microscope and transmission electron microscope, the leakage of intracellular contents was determined by ultraviolet spectrophotometry, and intracellular calcium ion concentration and nonspecific esterase activity were investigated by fluorescence spectroscopy. The results showed that the optimal pulsed light parameters were determined as 5 cm and 5 cycles/s for distance and flash frequency, respectively. Under the optimized conditions, the total number of bacterial colonies was killed by more than 50% and 99.98% at 15 and 40 s of irradiation time, respectively. The structures of the cell and spore wall were damaged, and intercellular nucleic acid, proteins, diethyl 2,6-pyridinedicarboxylate, Ca2+ and non-specific esterase activity were decreased after irradiation. The findings may provide a theoretical basis for the industrial application of pulsed light.

Key words: pulsed light, Bacillus subtilis, spore, inactivation mechanism

中图分类号:

TS201.3

管峰，徐雯钗，袁勇军，张瑞雪，赵思敏，陈秋平. 脉冲强光对枯草芽孢杆菌NG-2灭活机理[J]. 食品科学, 2017, 38(23): 70-74.

GUAN Feng, XU Wenchai, YUAN Yongjun, ZHANG Ruixue, ZHAO Simin, CHEN Qiuping. Inactivation and Mechanism of Bacillus subtilis NG-2 and Its Spores by Pulsed Light[J]. FOOD SCIENCE, 2017, 38(23): 70-74.

[1]	高兆建，秦宸锴，黄亮浩，朱双，朱劲虎，赵宜峰. 腊肉拮抗菌分离及抗真菌脂肽特性分析[J]. 食品科学, 2021, 42(8): 129-136.
[2]	王伟，李津津，迟海. 解淀粉芽孢杆菌素Amylocyclicin W5的纯化及其抑菌机理[J]. 食品科学, 2021, 42(7): 29-34.
[3]	任宏荣，李苗云，朱瑶迪，赵改名，赵莉君，吴慧琳，肖康，崔文明. 产气荚膜梭菌在食品中的危害及其控制研究进展[J]. 食品科学, 2021, 42(7): 352-359.
[4]	吴博华，蒋雪薇，张金玉，阮志强，梁胜男，陈永发，周尚庭. 促酱醪发酵芽孢杆菌的筛选及应用[J]. 食品科学, 2021, 42(6): 134-141.
[5]	陈茏，杨俊，马毛毛，吴莎莎，余平，曾哲灵. 产蛋白酶菌株的筛选及以菜籽粕为氮源的产酶条件优化[J]. 食品科学, 2021, 42(4): 115-121.
[6]	江君，刘军，杨绍青，马帅，闫巧娟，江正强. 黄杆菌褐藻胶裂解酶的高效表达及其在褐藻寡糖制备中的应用[J]. 食品科学, 2021, 42(4): 145-152.
[7]	高兆建，王秋芬，丁飞鸿，许祥，赵宜峰，焦魏，陈腾. 广谱拮抗菌株的筛选诱变及抗菌物质分离鉴定[J]. 食品科学, 2021, 42(2): 143-150.
[8]	高兆建，胡鑫强，宋玉林，丁飞鸿，赵宜峰，陈腾. 解淀粉芽孢杆菌I型耐热普鲁兰酶的纯化及酶特性分析[J]. 食品科学, 2021, 42(12): 130-137.
[9]	黄晓宁，刘晶晶，韩北忠，陈晶瑜. 基于酶学特性筛选大曲来源芽孢杆菌用于强化酿酒[J]. 食品科学, 2021, 42(10): 218-224.
[10]	陈宝莉，秦臻，赵黎明. 解淀粉芽孢杆菌β-N-乙酰氨基葡萄糖苷酶基因的克隆表达及其酶学表征[J]. 食品科学, 2020, 41(8): 123-129.
[11]	阮丽英，温志友，魏雪团. 解淀粉芽孢杆菌mtnN基因对其生物合成S-腺苷甲硫氨酸的影响[J]. 食品科学, 2020, 41(6): 193-200.
[12]	郭全友，刘玲，李保国，杨絮，姜朝军. Nisin、ɛ-聚赖氨酸、pH值对虾源枯草芽孢杆菌生长/非生长界面模型构建与评价[J]. 食品科学, 2020, 41(22): 140-147.
[13]	张妍，武俊瑞，曹承旭，邱博书，马颖，史海粟，杨慧，乌日娜. 芽孢杆菌对发酵大豆产生氨基酸和挥发性香气成分的影响[J]. 食品科学, 2020, 41(20): 242-248.
[14]	向慧平，林宜锦，关统伟，张家旭，尚红光，赵小林，焦士蓉，冯栩，杨阳. 四川浓香型大曲生产中酵母菌、芽孢杆菌与工艺指标的关联性分析[J]. 食品科学, 2020, 41(2): 196-201.
[15]	刘彦敏，沈璐，王康，严金平，伊日布斯. 传统大豆发酵食品中纳豆芽孢杆菌的分离及纳豆发酵[J]. 食品科学, 2020, 41(2): 208-214.

脉冲强光对枯草芽孢杆菌NG-2灭活机理

Inactivation and Mechanism of Bacillus subtilis NG-2 and Its Spores by Pulsed Light

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价