食品科学 ›› 2017, Vol. 38 ›› Issue (23): 135-140.doi: 10.7506/spkx1002-6630-201723022

• 营养卫生 • 上一篇    下一篇

基于转录组学技术探究新琼四糖对过度疲劳性肠道损伤的保护作用

宋 佳,张 娜,李 晶,毛相朝,薛长湖,唐庆娟*   

  1. 中国海洋大学食品科学与工程学院,山东 青岛 266003
  • 出版日期:2017-12-15 发布日期:2017-12-07
  • 基金资助:
    国家自然科学基金面上项目(31271923);国家自然科学基金联合基金项目(U1406402)

Protective Effect of Neoagarotetraose against Over-Exhaustion-Induced Gut Injury in Mice Revealed by RNA-Seq

SONG Jia, ZHANG Na, LI Jing, MAO Xiangzhao, XUE Changhu, TANG Qingjuan*   

  1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
  • Online:2017-12-15 Published:2017-12-07

摘要: 目的:运用转录组学探究新琼四糖对过度疲劳性肠道损伤的保护作用。方法:雄性BALB/c小鼠被随机 分为正常组、力竭运动组、阳性对照低聚果糖组和新琼四糖干预组,连续喂养16 d后,取小鼠结肠组织,应用 RNA-seq技术对结肠组织RNA进行完全测序,并对测序结果进行分析。结果:过度疲劳引起小鼠结肠肿瘤标 志物Zfp521和Zmynd8等锌指蛋白表达量极显著增加(P<0.01)、抑癌因子Casz1、Zfp346等的表达显著降低 (P<0.05),新琼四糖可缓解过度疲劳造成的肠道损伤,下调肿瘤相关锌指蛋白的表达,上调抑癌锌指蛋白的表 达。结论:新琼四糖可保护肠道、缓解过度疲劳引发的肠道损伤、预防肿瘤的发生。

关键词: 新琼四糖, 过度疲劳, 锌指蛋白, RNA-seq, 低聚果糖

Abstract: Purpose: To explore the protective effect of neoagarotetraose against over-exhaustion-induced gut injury by RNA-seq analysis. Methods: Male BALB/c mice were randomly divided into normal control group, over-exhaustion model group (both the groups were gavaged with saline), over-exhaustion plus fructooligosaccharide (FOS) group (positive control), and over-exhaustion plus neoagarotetraose (NAT) group. After sixteen days of administration, colon tissues from the mice in each group were collected, and then RNA was extracted for RNA-seq analysis. Results: Over-exhaustion resulted in significantly increased expression of zinc finger proteins Zfp521 and Zmynd8, associated with inflammation and tumor proliferation (P < 0.01), and significantly reduced expression of tumor suppressors Casz1 and Zfp346 (P < 0.05). NAT could relieve over-exhaustion-induced gut injury by downregulating the expression of zinc finger proteins related to tumor proliferation and upregulating the expression of tumor-suppressing zinc finger proteins. Conclusion: NAT can protect against over-exhaustion-induced gut injury in mice and prevent the occurrence of tumors.

Key words: neoagarotetraose, over-exhaustion, zinc finger protein, RNA-seq, fructooligosaccharide

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