食品科学 ›› 2018, Vol. 39 ›› Issue (2): 210-214.doi: 10.7506/spkx1002-6630-201802033

• 成分分析 • 上一篇    下一篇

基于拉曼光谱法测定冷鲜牛肉中肌红蛋白相对含量

张同刚,罗瑞明,李亚蕾*,周亚玲   

  1. (宁夏大学农学院,宁夏?银川 750021)
  • 出版日期:2018-01-25 发布日期:2018-01-05
  • 作者简介:张同刚,罗瑞明,李亚蕾,周亚玲
  • 基金资助:
    国家自然科学基金地区科学基金项目(31660442)

Determination of Myoglobin in Chilled Beef Based on Raman Spectroscopy

ZHANG Tonggang, LUO Ruiming, LI Yalei*, ZHOU Yaling   

  1. (School of Agriculture, Ningxia University, Yinchuan 750021, China)
  • Online:2018-01-25 Published:2018-01-05

摘要: 为研究4 ℃贮藏期间冷鲜牛肉3?种化学形式肌红蛋白相对含量变化,对采集的一维拉曼光谱,用2D-shige二维相关分析软件对其光谱数据进行二维相关分析,得出3?种化学形式肌红蛋白的相对含量最佳推导公式。结果表明:肌红蛋白、氧合肌红蛋白与高铁肌红蛋白相对含量的最佳推导公式分别为:脱氧肌红蛋白相对含量/%=(-0.305?5x+19.570)×100;氧合肌红蛋白相对含量/%=(-2.933?9x+154.17)×100;高铁肌红蛋白相对含量/%=(1.574?3x-14.369)×100。3?种化学形式的肌红蛋白相对含量的预测效果为高铁肌红蛋白>脱氧肌红蛋白>氧合肌红蛋白,其中波数为1?033~1?248?cm-1时,高铁肌红蛋白相对含量的误差值最小为4.76%。研究表明,基于拉曼光谱法无损检测冷鲜牛肉中肌红蛋白相对含量是可行的。?

关键词: 拉曼光谱, 二维相关光谱技术, 冷鲜牛肉, 肌红蛋白

Abstract: In this study, we examined the changes in the relative amounts of three chemical forms of myoglobin, deoxymyoglobin (DeoxyMb), oxymyoglobin?(OxyMb) and metmyoglobin (MetMb), in fresh beef meat stored at 4 ℃. For this purpose, one-dimensional Raman spectral data were acquired and analyzed by two-dimensional correlation analysis using 2D-shige software. The optimal formulas for the relative amounts of three chemical forms of Mb were DeoxyMb/% = (?0.305 5x + 19.570) × 100; OxyMb/% = (?2.933 9x + 154.17) × 100; and MetMb/% = (1.574 3x ? 14.369) × 100, respectively. The prediction efficiency of these formulas was in the decreasing order: MetMb > DeoxyMb > OxyMb. When the Raman frequency was 1 033–1 248 cm-1, the root mean square error of prediction of MetMb was minimum, only 4.76%. Therefore, it is feasible to determine the relative amount of myoglobin using Raman spectroscopy.

Key words: Raman spectroscopy, two-dimensional correlation spectroscopy, chilled beef, myoglobin

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