关键词: 超高效液相色谱-四极杆飞行时间质谱, 水产品, QuEChERS, 激素

Abstract: An analytical method was established for rapid screening of 16 hormone residues in aquatic product by modified QuECHERS combined with ultra performance liquid Chromatography-quadrupole time of flight mass spectrometry (UPLC-QTOF-MS)．The homogenized sample were extracted with 1% acetic acid acetonitrile，salting out with sodium chloride and anhydrous magnesium sulfate，and defatted with n-hexane，then cleaned-up by C18．Analysis were separated on an BEH C18 column using water with 0.1% formic acid and methanol as mobile phase by gradient，analyzed by MS under ESI positive ionization mode．The linear range was 1～100μg/L for each compound with the correlation coefficients of more than 0.9906. At the different spiked level，the recoveries were ranged from 53.6% to 135.2%，with relative standard deviations (RSDs) ranged from 0.93% to10.93% in Penaeus vannamei，Pseudosciaena crocea，Grass Carp and Swimming crab matrices。The limits of detection (LODs) （S/N=3） were 0.2～2.7μg/kg and the limits of quantification (LOQs) （S/N=10） for the analytes were 0.8～9.0μg/kg. The method is simple, rapid and accurate for rapid screening of hormones in aquatic products samples．

Key words: ultra performance liquid Chromatography-quadrupole time of flight mass spectrometry (UPLC-QTOF-MS), aquatic product, QuEChERS, hormones