食品科学 ›› 2018, Vol. 39 ›› Issue (3): 41-48.doi: 10.7506/spkx1002-6630-201803007

• 基础研究 • 上一篇    下一篇

啤酒花废弃枝叶多酚、黄酮含量与抗氧化活性的相关性分析

白姗姗,张莹莹,赵 强,刘玉梅*   

  1. 新疆大学化学化工学院,煤炭清洁转化与化工过程自治区重点实验室,新疆 乌鲁木齐 830046
  • 出版日期:2018-02-15 发布日期:2018-01-30
  • 基金资助:
    国家自然科学基金地区科学基金项目(31360403)

Correlation of Antioxidant Properties with Contents of Total Polyphenols and Total Flavonoids in Successive Solvent Extracts of Abandoned Hop Branches and Leaves

BAI Shanshan, ZHANG Yingying, ZHAO Qiang, LIU Yumei*   

  1. Key Laboratory of Coal Cleaning Conversion and Chemical Engineering Process, Xinjiang Uyghur Autonomous Region, College of Chemistry and Chemical Engineering, Xinjiang University, ürümqi 830046, China
  • Online:2018-02-15 Published:2018-01-30

摘要: 采用不同极性溶剂对新疆种植的札一和马可波罗啤酒花废弃枝叶的乙醇提取物进行不同极性部位的分 离,以羟自由基、1,1-二苯基-2-三硝基苯肼自由基、β-胡萝卜素-亚油酸及磷钼酸4 个不同的抗氧化活性评价体系 对获得的各提取物的抗氧化活性进行比较性研究,并与提取物中多酚、黄酮含量进行相关性分析。结果表明, 乙醇提取物及各极性部位均具有一定的抗氧化性。其中,乙酸乙酯提取物中的多酚和黄酮的含量均最高,其在主 枝、侧枝和叶子的含量范围分别为5.80~34.20 mg/g和2.00~18.00 mg/g;水相中最低,上述各物质的含量范围仅为 0.73~11.05 mg/g和0.06~5.74 mg/g。相关性分析表明,啤酒花主枝、侧枝和叶子抗氧化活性与其多酚、黄酮含量 具有显著的相关关系(P<0.05)。

关键词: 啤酒花, 废弃枝叶, 多酚, 黄酮, 抗氧化性, 相关性

Abstract: The ethanol extracts of abandoned hop branches and leaves of SA-1 and Marco Polo hop varieties grown in Xinjiang were separated by using different polar solvents. The antioxidant activities of the resulting extracts were studied by four in vitro assays: hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl radical, β-carotene-linoleic acid and phosphomolybdenum model systems and were correlated with the contents of polyphenols and flavonoids. The results showed that all samples displayed antioxidant activity. Ethyl acetate extracts from hop main stems, side stems, and leaves had the highest contents of phenols and flavonoids, which were 5.80–34.20 and 2.00–18.00 mg/g, respectively. On the contrary, water extracts had the lowest contents of phenols and flavonoids, which were 0.73–11.05 and 0.06–5.74 mg/g, respectively. The correlation analysis showed that hop main stems, side stems and leaves exhibited significant relationship between antioxidant activity and the contents of phenols and flavonoids (P < 0.05).

Key words: hop, abandoned branches and leaves, polyphenols, flavonoids, antioxidant activity, correlation

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