• 生物工程 •

### 岩藻低聚糖的酶法制备及活性分析

1. （1.青岛农业大学食品学院，山东青岛 266109；2.浙江经贸职业技术学院，浙江杭州 310018）
• 出版日期:2018-02-25 发布日期:2018-02-02
• 基金资助:
山东省高等学校科技计划项目（J15LE13）；青岛农业大学高层次人才启动基金项目（6631113347）

### Enzymatic Preparation and Activity of Fucosylated Oligosaccharides

WANG Fengwu1, QUE Fei2, KOU Lingyun1, CHI Fang1, WANG Ying1,*

1. (1. College of Food, Qingdao Agricultural University, Qingdao 266109, China; 2. Zhejiang Institute of Economics and Trade, Hangzhou 310018, China)
• Online:2018-02-25 Published:2018-02-02

Abstract: Fucoidanase was used to degrade fucoidan to obtain fucosylated oligosaccharides. A total of 122 experiments were designed and implemented using uniform design and orthogonal array design. A three-layer BP network model was built to simulate the hydrolysis process, and it was optimized by genetic algorithm using Matlab software. A mouse model of D-galactose-induced aging was used to test the antioxidant activities of fucosylated oligosaccharides and fucoidan. The results showed that the molecular weight of hydrolysates was changed significantly during 4 h hydrolysis at 20–40 ℃ with 0–24 U/mg of fucoidanase. On the basis of this finding, the optimization was carried out and the result showed that an enzyme dosage of 20.7 U/mg, a temperature of 26 ℃ and a hydrolysis time of 2.3 h were found to be the optimal conditions to obtain a hydrolysate with the highest antioxidant activity. The hydrolysate scavenged 85.1% 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical with a molecular weight of 18.2 kDa. The results of animal experiments showed that the antioxidant activity of fucosylated oligosaccharides were significantly higher than that of fucoidan in terms of hydroxyl free radical scavenging activity, catalase (CAT) activity and malonaldehyde (MDA) content (P < 0.05). This paper can provide experimental support for the industrial preparation of fucoidan hydrolyzate with high antioxidant activity.